In-house SIRAS phasing of the polyunsaturated fatty-acid isomerase from Propionibacterium acnes

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Alena LiavonchankaMarkus Georg Rudolph

Abstract

The polyenoic fatty-acid isomerase from Propionibacterium acnes (PAI) catalyzes the double-bond isomerization of linoleic acid to conjugated linoleic acid, which is a dairy- or meat-derived fatty acid in the human diet. PAI was overproduced in Escherichia coli and purified to homogeneity as a yellow-coloured protein. The nature of the bound cofactor was analyzed by absorption and fluorescence spectroscopy. Single crystals of PAI were obtained in two crystal forms. Cubic shaped crystals belong to space group I2(1)3, with a unit-cell parameter of 160.4 A, and plate-like crystals belong to the monoclinic space group C2, with unit-cell parameters a = 133.7, b = 60.8, c = 72.2 A, beta = 115.8 degrees. Both crystal forms contain one molecule per asymmetric unit and diffract to a resolution of better than 2.0 A. Initial phases were obtained by SIRAS from in-house data from a cubic crystal that was soaked with an unusually low KI concentration of 0.25 M.

References

Feb 10, 2000·Acta Crystallographica. Section D, Biological Crystallography·Z DauterK R Rajashankar
Jun 9, 2001·Biochemical and Biophysical Research Communications·Y ChoiJ M Ntambi
Sep 27, 2001·Nature Structural Biology·D A SandersJ H Naismith
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider, George M Sheldrick
Dec 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Isabel UsónGeorge M Sheldrick
Nov 4, 2004·Progress in Lipid Research·Klaus W J WahleDino Rotondo
Oct 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·P H Zwart

❮ Previous
Next ❯

Citations

Nov 24, 2006·Lipids·Sibel IrmakMichael Eisenmenger
Jan 24, 2009·The Journal of Biological Chemistry·Alena LiavonchankaIvo Feussner
Jul 16, 2013·Bioengineered·Kerry Joan O'ConnellDouwe van Sinderen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.