In human cell cultures, everolimus is inferior to tacrolimus in inhibiting cellular alloimmunity, but equally effective as regards humoral alloimmunity

International Urology and Nephrology
Theodoros EleftheriadisIoannis Stefanidis

Abstract

Acute cellular rejection is the major cause of immune-mediated graft failure early in the course of kidney transplantation, whereas chronic antibody-mediated rejection is a major contributor to graft loss in the late post-transplant phase. Based mainly on the results of short-term studies, the calcineurin inhibitor tacrolimus prevails over the mammalian target of rapamycin (mTOR) inhibitors. However, the toxicity profile of the two drug categories differs, making the interchange between them appealing. In this study, the effect of tacrolimus and of the mTOR inhibitor everolimus on cellular and humoral alloimmunity was evaluated. Cellular alloimmunity was assessed by cell proliferation in two-way mixed lymphocyte reaction (MLR) with human peripheral blood mononuclear cells (PBMC). For assessing humoral alloimmunity, we developed a method in which humoral alloimmunity was induced in a one-way MLR. The de novo production of alloantibodies was measured with an antibody-mediated complement-dependent cytotoxicity assay, in which supernatants from the above MLRs were used against resting PBMC similar to the stimulator cells of the forementioned MLRs. Tacrolimus and everolimus were used at concentrations near their upper recommended tr...Continue Reading

References

Sep 13, 1991·Journal of Immunological Methods·N W RoehmA L Glasebrook
Jan 1, 1993·Journal of Pharmaceutical Sciences·D P Wang, M K Yeh
Feb 14, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Herwig-Ulf Meier-KriescheBruce Kaplan
Dec 21, 2007·The New England Journal of Medicine·Henrik EkbergUNKNOWN ELITE-Symphony Study
Nov 20, 2009·Clinical and Experimental Immunology·S HeidtA Mulder
Jan 29, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Henrik EkbergPavel Navrátil
Jul 21, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L MjörnstedtH Holdaas
Nov 28, 2013·Transplant International : Official Journal of the European Society for Organ Transplantation·Steven J ChadbanUNKNOWN SOCRATES Study Group
Jul 16, 2014·Annals of Transplantation : Quarterly of the Polish Transplantation Society·Lionel RostaingJulio Pascual
Apr 26, 2015·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Donald E Hricik
Feb 7, 2016·Clinical Biochemistry·Luis Eduardo BeckerCaner Suesal
Apr 2, 2016·International Journal of Molecular Medicine·Theodoros EleftheriadisIoannis Stefanidis

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Citations

Oct 5, 2020·Archivum Immunologiae Et Therapiae Experimentalis·Theodoros EleftheriadisIoannis Stefanidis
Jan 20, 2019·Bio-protocol·Georgios Pissas, Theodoros Eleftheriadis
Jan 16, 2019··Theodoros EleftheriadisGeorgios Pissas

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