PMID: 6969751Feb 1, 1981Paper

In in vitro production and cloning of the P cell, a bone marrow-derived null cell that expresses H-2 and Ia-antigens, has mast cell-like granules, and is regulated by a factor released by activated T cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
J W Schrader

Abstract

A technique, based on selective culture conditions, is described for the preparation from mouse spleen of a pure population of Ig-negative, Thy.1-negative and Lyt.1- and Lyt.2-negative nonadherent cells, termed persisting (P) cells. Antigens coded for by the H2-K, H2-D, and I regions were readily detectable on the surface of P cells. P cells grew for long periods in vitro provided that a factor produced by activated T cells, but distinct from T cell growth factor, was present. It was demonstrated that P cells were bone marrow derived. When P cells were examined in suspension, a variable number of fine cytoplasmic projections could be seen waving from the cell, often at 1 pole. The nuclei varied in shape, being round, in general, but with bilobed and irregular forms occurring, and the cytoplasm contained large numbers of characteristic metachromatic granules. P cells had readily detectable Fc receptors but did not phagocytose latex particles or antibody-coated erythrocytes. P cells formed colonies in soft agar in the presence of growth factors, and cloned lines could be propagated from these colonies. The relationship of P cells to mast cells and Ia-positive accessory cells and their possible role in T cell activation is discussed.

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.