In multiple myeloma clonotypic CD38- /CD19+ / CD27+ memory B cells recirculate through bone marrow, peripheral blood and lymph nodes

Leukemia & Lymphoma
T RasmussenHans Erik Johnsen

Abstract

It is believed that myeloma cells are derived from a germinal center (GC) or post GC B cell. The GC B cell can differentiate into both a memory B cell and a plasma cell (PC). In this study, we investigated the recirculating potential of memory B cells clonally related to the myeloma PC (termed clonotypic). The V(H)DJ(H) immunoglobulin gene rearrangement of the myeloma clone was identified for 10 myeloma patients and allele-specific oligonucleotides (ASO) IgH RT-PCR assays were designed for each patient. Memory B cells (CD38- /CD19+ /CD27+) and their subsets defined by the monoclonal antibodies CD62L, CCR6, CXCR4, CXCR5, CCR7 were flow-sorted as single cells and analyzed by ASO RT-PCR analysis. In addition, aspirated peripheral lymph nodes (PLN) of 7 myeloma patients in complete or partial remission were analyzed for the presence of clonotypic cells. Circulating clonotypic memory B cells were identified in PBMNC of 7/10 patients and both CD62L positive and negative clonotypic memory B cells were identified. Furthermore, comparable frequencies of clonotypic cells were found in the CCR6 +/- and CXCR4 +/- memory B cell subsets, whereas all clonotypic memory and later stage B cells were CXCR5 positive. In accordance with their immun...Continue Reading

References

Jan 1, 1993·Annual Review of Immunology·D Gray
Apr 27, 2000·Immunology Today·K AgematsuA Komiyama
Jan 6, 2001·Acta Oncologica·T RasmussenH E Johnsen
Jul 4, 2001·The Journal of Experimental Medicine·D C HargreavesJ G Cyster

❮ Previous
Next ❯

Citations

Sep 19, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kelly BoucherLia E Perez
Jun 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Carol Ann Huff, William Matsui
Apr 5, 2013·International Journal of Clinical Oncology·Sung Hak LeeChang Suk Kang
Dec 10, 2013·Journal of Hematology & Oncology·Joshua KellnerZihai Li
Sep 4, 2013·Leukemia & Lymphoma·Hans Erik JohnsenUNKNOWN Myeloma Stem Cell Network (MSCNET)
Feb 27, 2010·Critical Reviews in Oncology/hematology·Axel SchulenburgPeter Valent
Sep 25, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Hans Erik JohnsenKaren Dybkaer
Nov 13, 2007·Hematology/oncology Clinics of North America·Constantine S MitsiadesKenneth C Anderson
Jun 10, 2010·Cytometry. Part B, Clinical Cytometry·Hans E JohnsenUNKNOWN Myeloma Stem Cell Network (MSCNET)
Apr 12, 2013·Clinical and Experimental Immunology·L A AqrawiK A Brokstad
Oct 12, 2013·British Journal of Haematology·Roman HajekHana Svachova
Aug 26, 2015·Stem Cells·Anastasios KaradimitrisXiaolin Xiao
Nov 10, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Grzegorz Wladyslaw Basak, Ewa Carrier
Dec 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kilian Wistuba-HamprechtGraham Pawelec
Sep 5, 2008·Current Protocols in Cytometry·Andy C Rawstron
Jun 8, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gautam N ShenoyAnna George

❮ Previous
Next ❯

Related Concepts

Related Feeds

CNS Lymphoma

In CNS lymphoma, cancerous cells from lymph tissues or other parts of the body form tumors in the brain and/or spinal cord. Here is the latest research on this rare non-Hodgkin lymphoma.

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.