In-ovo exposed carbon black nanoparticles altered mRNA gene transcripts of antioxidants, proinflammatory and apoptotic pathways in the brain of chicken embryos

Chemico-biological Interactions
Dalia H SamakMasakazu Umezawa

Abstract

With ubiquitous applications of nanotechnology, there are increasing probabilities of exposure to manufactured nanoparticles (NPs), which might be posing emerging health concerns on the next generation. Recent data suggest that generation of reactive oxygen species may play an integral role in the carbon black nanoparticles (CBNPs)-induced oxidative injury; however, the exact molecular mechanism has not been clarified. Hence, the role of oxidative stress, inflammation and apoptosis pathways in the CBNPs-induced neuronal toxicity following in-ovo exposure of chicken embryo was elucidated. Specific pathogen-free fertilized Sasso eggs were inoculated with 4.8, 9.5 and 14 μg CBNPs/egg at the 3rd day of incubation alongside vehicle controls. In a concentration-dependent manner, CBNPs inoculation induced oxidative stress, which was ascertained by enhancement of lipid peroxides and diminishing total antioxidant capacity and glutathione levels, and catalase activity in brain tissues. mRNA transcript levels of antioxidant genes showed up-regulation of heme oxygenase-1 and superoxide dismutase-1, with marked down-regulation of glutathione S-transferase-α. Additionally, the pro-inflammatory genes; nuclear factor-κB1 was up-regulated, whil...Continue Reading

Citations

Apr 25, 2019·Particle and Fibre Toxicology·Michael RiedikerFlemming Cassee
Dec 1, 2018·Environmental Science and Pollution Research International·Dalia H SamakMohamed M Abdel-Daim
Aug 9, 2019·Environmental Science and Pollution Research International·Mohamed M A HusseinIslam M Saadeldin
May 27, 2021·Environmental Science and Pollution Research International·Shimaa M Abou-ZeidNermeen B El-Borai
Aug 28, 2021·Medical Sciences : Open Access Journal·Eberhard GrambowDaniel Strüder

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