In ovo gene manipulation of melanocytes and their adjacent keratinocytes during skin pigmentation of chicken embryos

Development, Growth & Differentiation
Hidetaka MuraiYoshiko Takahashi

Abstract

During skin pigmentation in avians and mammalians, melanin is synthesized in the melanocytes, and subsequently transferred to adjacently located keratinocytes, leading to a wide coverage of the body surface by melanin-containing cells. The behavior of melanocytes is influenced by keratinocytes shown mostly by in vitro studies. However, it has poorly been investigated how such intercellular cross-talk is regulated in vivo because of a lack of suitable experimental models. Using chicken embryos, we developed a method that enables in vivo gene manipulations of melanocytes and keratinocytes, where these cells are separately labeled by different genes. Two types of gene transfer techniques were combined: one was a retrovirus-mediated gene infection into the skin/keratinocytes, and the other was the in ovo DNA electroporation into neural crest cells, the origin of melanocytes. Since the Replication-Competent Avian sarcoma-leukosis virus long terminal repeat with Splice acceptor (RCAS) infection was available only for the White leghorn strain showing little pigmentation, melanocytes prepared from the Hypeco nera (pigmented) were back-transplanted into embryos of White leghorn. Prior to the transplantation, enhanced green fluorescent p...Continue Reading

References

Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z Abdel-MalekV J Hearing
Jun 3, 1998·Methods : a Companion to Methods in Enzymology·M Logan, C Tabin
Aug 13, 1999·Development, Growth & Differentiation·J FunahashiH Nakamura
Sep 6, 2002·Pigment Cell Research·Glynis Scott
Jul 16, 2003·Pigment Cell Research·Dorothy C Bennett, M Lynn Lamoreux
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Paul M KulesaMary J C Hendrix
Jun 22, 2006·Traffic·Karolien Van Den BosscheJo Lambert
Jul 4, 2006·Methods : a Companion to Methods in Enzymology·Tomoya KotaniKoichi Kawakami
Mar 17, 2007·Developmental Biology·Yuki SatoYoshiko Takahashi
Jan 6, 2009·International Review of Cell and Molecular Biology·Sara AspengrenMargareta Wallin
Mar 9, 2011·PloS One·Bernadett KormosZsuzsanna Bata-Csörgo

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Citations

Mar 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Masafumi InabaCheng Ming Chuong
Feb 17, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Haruka MatsubaraKoji Tamura
Dec 23, 2016·The Journal of Biological Chemistry·Jaroslaw SzczyrbaGunther Wennemuth

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