In ovo oral vaccination with Campylobacter jejuni establishes early development of intestinal immunity in chickens

British Poultry Science
S M NoorP R Widders

Abstract

1. Chick embryos were orally immunised at day 16 of incubation by injection of heat-killed Campylobacter jejuni organisms into the amniotic fluid. The response to vaccination was observed at 5 d after hatching or, in some birds which received a postnatal oral booster vaccination, at 7 d after hatching, and the response was observed at 14 d of age. 2. The titres of antibody in serum, bile and intestinal scrapings, the distribution of immunoglobulin-containing cells in the spleen, duodenum and ileum and the expression on peripheral blood leukocytes (PBL) of the T cell surface markers CD3, CD4 and CD8 were determined. 3. Whereas low titres of anti-flagellin antibody were detected in serum, bile and intestinal scrapings of unimmunised birds, high titres were observed in immunised birds. 4. An increase in antibody of all isotypes was detectable in serum but the elevation in IgA antibody in intestinal scrapings and bile was particularly striking. This response was reflected in a dramatic increase in immunoglobulin-containing cells, detected by fluorescent histology, particularly those associated with IgA and IgM isotypes in the spleen and intestine of immunised birds. 5. Secondary oral boosting after hatching resulted in a depression...Continue Reading

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Citations

Mar 16, 2000·Developmental and Comparative Immunology·W I MuirA J Husband
Sep 1, 1996·British Poultry Science·P R WiddersK A Long
Aug 7, 2003·Applied and Environmental Microbiology·D G Newell, C Fearnley
Jun 1, 2016·Frontiers in Microbiology·Patrycja A KobiereckaElżbieta K Jagusztyn-Krynicka
Nov 28, 2017·PloS One·Marine MeunierMarianne Chemaly
Jan 25, 2011·Microbial Biotechnology·Byeonghwa JeonQijing Zhang
Apr 9, 2020·Avian Diseases·Julia K SchmiedekeUlrike Blohm
May 22, 2020·Bulletin of the National Research Centre·Nagwa S Rabie, Zeinab M S Amin Girh
Nov 17, 2020·Frontiers in Veterinary Science·Graham A J RedweikMelha Mellata
Jan 17, 2007·Vaccine·Marcel R de ZoeteJaap A Wagenaar

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