In planta comparative transcriptomics of host-adapted strains of Ralstonia solanacearum

PeerJ
Florent AilloudPhilippe Prior

Abstract

Background. Ralstonia solanacearum is an economically important plant pathogen with an unusually large host range. The Moko (banana) and NPB (not pathogenic to banana) strain groups are closely related but are adapted to distinct hosts. Previous comparative genomics studies uncovered very few differences that could account for the host range difference between these pathotypes. To better understand the basis of this host specificity, we used RNAseq to profile the transcriptomes of an R. solanacearum Moko strain and an NPB strain under in vitro and in planta conditions. Results. RNAs were sequenced from bacteria grown in rich and minimal media, and from bacteria extracted from mid-stage infected tomato, banana and melon plants. We computed differential expression between each pair of conditions to identify constitutive and host-specific gene expression differences between Moko and NPB. We found that type III secreted effectors were globally up-regulated upon plant cell contact in the NPB strain compared with the Moko strain. Genes encoding siderophore biosynthesis and nitrogen assimilation genes were highly up-regulated in the NPB strain during melon pathogenesis, while denitrification genes were up-regulated in the Moko strain ...Continue Reading

References

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Citations

Nov 24, 2016·Environmental Microbiology·Simon LeonardSylvie Reverchon
Jan 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shihao SongYinyue Deng
May 8, 2020·Microbial Genomics·Osiel Silva GonçalvesMateus Ferreira Santana
Apr 5, 2017·Frontiers in Plant Science·Marina PuigvertMarc Valls

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Datasets Mentioned

BETA
GMI1000
PRJNA297400
PRJNA297402

Methods Mentioned

BETA
transgenic

Software Mentioned

Bedtools
FastQC
DEseq2
HGAP
SMRT Portal
edgeR
Bowtie2
R
PALOMA
Trimmomatic

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