PMID: 9421494Feb 28, 1998Paper

In planta transcription of a second subgenomic RNA increases the complexity of the subgroup 2 luteovirus genome

Nucleic Acids Research
A AshoubD Prüfer

Abstract

The genetic information of potato leafroll virus (PLRV), a typical member of the subgroup 2 luteoviruses, is contained in a single-stranded (+) sense RNA of approximately 5.9 kb. A single subgenomic RNA (sgRNA1) of approximately 2.3 kb has been characterized as the mRNA for the 3' clustered viral open reading frames ORF3, ORF3/5 and ORF4. Here we demonstrate by Northern blot analyses of polysomal RNAs from PLRV-infected Solanum tuberosum and Physalis floridana plants that, as with luteoviruses belonging to subgroup 1, in planta synthesis of a second 0.8 kb subgenomic RNA (sgRNA2) increases the complexity of subgroup 2 luteoviral genomes significantly. PLRV-specific hybridization probes as well as primer extension experiments map sgRNA2 to the 3'-end of the PLRV RNA genome (positions 5190-5987). Similarly, for the closely related cucurbit aphid-borne yellows virus (CABYV) a sgRNA2 of similar size and position (positions 4888-5669) was identified. PLRV sgRNA2 may code for two viral proteins of 7.1 (ORF6) and 14 kDa (ORF7) respectively, while the CABYV proteins are 8.7 (ORF6) and 8.3 kDa (ORF7) in size, with PLRV ORF7 displaying nucleic acid binding activity. In vivo experiments by transient expression of chimeric GUS fusions in p...Continue Reading

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Citations

Aug 21, 2012·Virus Genes·Shaheen Nourinejhad ZarghaniMaghsoud Pazhouhandeh
Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·A JeevalathaB P Singh
Jan 1, 2005·Molecular Plant Pathology·Mark StevensOlivier Lemaire
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hannah Miriam JaagDirk Prüfer
Mar 1, 2003·Molecular Plant Pathology·Michael TalianskyHugh Barker
Jun 16, 2015·Phytopathology·Mona A KassemMiguel A Aranda
Dec 20, 2005·Virus Research·Elizabeth L Pettit KnellerW Allen Miller
Jun 21, 2002·The Journal of General Virology·Leslie L DomierCleora J D'Arcy
Jan 1, 2014·Journal of Virological Methods·Leonidas LotosNikolaos I Katis
Nov 9, 2018·Frontiers in Microbiology·Diane Bortolamiol-BécetVéronique Ziegler-Graff
Feb 17, 2000·The Journal of General Virology·K M NurkiyanovaM E Taliansky
Jun 5, 2017·Virus Genes·João Marcos Fagundes SilvaThor Vinícius Martins Fajardo
Jun 22, 2000·The Journal of General Virology·X LiJ W Lamb

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