In Search of a Murine Model of Radiation-Induced Periprosthetic Capsular Fibrosis

Annals of Plastic Surgery
Alexandra J LinJason A Spector

Abstract

Capsular contracture after breast reconstruction is a morbid complication, occurring in 30.0% to 47.5% of patients undergoing postoperative radiotherapy. Although it is well known that radiation increases rate of capsular contracture, there are few well-established animal models that faithfully replicate standard-of-care clinical practice, that is, prosthesis placement at the time of mastectomy followed by delayed radiotherapy. To better recapitulate current clinical practice, we developed a murine model in which the implant sites were irradiated 10 days postoperatively, rather than at time of surgery. Hemispherical implants were created from polydimethylsiloxane and implanted bilaterally in the subcutaneous dorsa of 20 C57Bl/6 mice. Mice were randomized to 5 treatment groups, differing in irradiation dose: 0 to 40 Gy. Ten days postoperatively, irradiation was performed using 250-kVp x-rays (XRAD225Cx, Precision X-ray, North Branford, Conn). In 1 mouse per group, dosimeters were placed subcutaneously to measure the delivered absorbed dose. Thirty-one days postoperatively, the mice were sacrificed and examined grossly, and periprosthetic tissues were removed for histologic analysis of periprosthetic capsule thickness and cellula...Continue Reading

References

Mar 6, 1997·The New England Journal of Medicine·S E GabrielL J Melton
Mar 14, 2002·The American Journal of Pathology·Kathleen C FlandersAnita B Roberts
Oct 20, 2005·The Breast : Official Journal of the European Society of Mastology·E EltzeB Pfleiderer
Mar 10, 2006·Plastic and Reconstructive Surgery·Neal HandelJ Arthur Jensen
Apr 4, 2006·Plastic and Reconstructive Surgery·William P AdamsSpencer A Brown
Dec 29, 2007·Seminars in Immunology·James M AndersonDavid T Chang
Jul 3, 2008·Plastic and Reconstructive Surgery·Stephen R SullivanF Frank Isik
Nov 4, 2008·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Gillian A WhitfieldCharles B Wilson
Mar 26, 2009·Plastic and Reconstructive Surgery·Ewa Komorowska-TimekDuncan A G Miles
Dec 3, 2009·Plastic and Reconstructive Surgery·Antonino AracoGianpiero Gravante
Nov 3, 2010·Plastic and Reconstructive Surgery·Evan B KatzelHoward N Langstein
Apr 20, 2011·Plastic and Reconstructive Surgery·Steven M SultanAlexes Hazen
May 28, 2011·Plastic and Reconstructive Surgery·Evan B KatzelHoward N Langstein
Sep 27, 2015·Clinics in Plastic Surgery·Simon J Chong, Anand K Deva
Oct 1, 2015·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Katherine E MillerJose Leiva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.