In silicio identification of glycosyl-phosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae

Yeast
L H CaroF M Klis

Abstract

Use of the Von Heijne algorithm allowed the identification of 686 open reading frames (ORFs) in the genome of Saccharomyces cerevisiae that encode proteins with a potential N-terminal signal sequence for entering the secretory pathway. On further analysis, 51 of these proteins contain a potential glycosyl-phosphatidylinositol (GPI)-attachment signal. Seven additional ORFs were found to belong to this group. Upon examination of the possible GPI-attachment sites, it was found that in yeast the most probable amino acids for GPI-attachment as asparagine and glycine. In yeast, GPI-proteins are found at the cell surface, either attached to the plasma-membrane or as an intrinsic part of the cell wall. It was noted that plasma-membrane GPI-proteins possess a dibasic residue motif just before their predicted GPI-attachment site. Based on this, and on homologies between proteins, families of plasma-membrane and cell wall proteins were assigned, revealing 20 potential plasma-membrane and 38 potential cell wall proteins. For members of three plasma-membrane protein families, a function has been described. On the other hand, most of the cell wall proteins seem to be structural components of the wall, responsive to different growth condition...Continue Reading

References

Aug 1, 1990·Trends in Biochemical Sciences·N Jentoft
Jul 16, 1986·Biochemical and Biophysical Research Communications·D Marguet, G J Lauquin
Jul 1, 1987·Molecular and Cellular Biology·A Percival-Smith, J Segall
Jun 11, 1986·Nucleic Acids Research·G von Heijne
May 28, 1985·Biochimica Et Biophysica Acta·P KleinC DeLisi
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Komano, R S Fuller
Nov 15, 1995·Molecular & General Genetics : MGG·T Roemer, H Bussey
Jan 1, 1995·Annual Review of Biochemistry·S Udenfriend, K Kodukula
May 1, 1995·The Journal of Cell Biology·D HamburgerH Riezman
Jun 1, 1995·Journal of Bacteriology·J M van der VaartC T Verrips
Jan 20, 1995·The Journal of Biological Chemistry·R KollárE Cabib
Mar 1, 1994·Current Genetics·F BidardP Barre
Jul 1, 1994·Yeast·F M Klis
Jul 1, 1996·Molecular and Cellular Biology·B KovacechT Schuster
Sep 1, 1996·Cell Biochemistry and Function·M AhmadzadehM Colombini
Dec 1, 1996·Journal of Bacteriology·W S Lo, A M Dranginis

❮ Previous
Next ❯

Citations

Aug 13, 2003·Eukaryotic Cell·Elisabetta SpreghiniAaron P Mitchell
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·B GuoG R Fink
Mar 8, 2000·Bioscience, Biotechnology, and Biochemistry·K YodaM Yamasaki
Apr 28, 2001·Journal of Bacteriology·M RichardC Gaillardin
Oct 13, 2001·Molecular Biology of the Cell·P FraeringA Conzelmann
Mar 28, 2002·Applied and Environmental Microbiology·Hitoshi ShimoiKiyoshi Ito
Oct 31, 2002·Applied and Environmental Microbiology·Ilaria MannazzuFrancesca Clementi
Nov 29, 2002·Eukaryotic Cell·Mingliang ZhangScott E Erdman
Jan 11, 2000·Clinical Microbiology Reviews·M A Ghannoum
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Julian R NaglikBernhard Hube
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
May 5, 2006·Molecular Biology of the Cell·Gertien J SmitsFrans M Klis
May 5, 2006·Applied and Environmental Microbiology·Sandrine ChabaneJean-Paul Latgé
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Dec 26, 2006·Eukaryotic Cell·Mathias L Richard, Armêl Plaine
Sep 1, 2007·Molecular Biology of the Cell·Mariko UmemuraYoshifumi Jigami
Oct 24, 2007·Eukaryotic Cell·Todd B ReynoldsGerald R Fink
Aug 25, 2009·Applied and Environmental Microbiology·Debra RossouwFlorian F Bauer
Aug 16, 2011·Eukaryotic Cell·Anita BoisraméMathias L Richard
Nov 6, 2001·American Journal of Respiratory Cell and Molecular Biology·R J Palmer, A E Wakefield

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.