In silicio search for genes encoding peroxisomal proteins in Saccharomyces cerevisiae

Cell Biochemistry and Biophysics
A J KalH F Tabak

Abstract

The biogenesis of peroxisomes involves the synthesis of new proteins that after, completion of translation, are targeted to the organelle by virtue of peroxisomal targeting signals (PTS). Two types of PTSs have been well characterized for import of matrix proteins (PTS1 and PTS2). Induction of the genes encoding these matrix proteins takes place in oleate-containing medium and is mediated via an oleate response element (ORE) present in the region preceding these genes. The authors have searched the yeast genome for OREs preceding open reading frames (ORFs), and for ORFs that contain either a PTS1 or PTS2. Of the ORFs containing an ORE, as well as either a PTS1 or a PTS2, many were known to encode bona fide peroxisomal matrix proteins. In addition, candidate genes were identified as encoding putative new peroxisomal proteins. For one case, subcellular location studies validated the in silicio prediction. This gene encodes a new peroxisomal thioesterase.

Citations

Nov 23, 2013·Nature Reviews. Molecular Cell Biology·Jennifer J Smith, John D Aitchison
Apr 3, 2012·Biochimica Et Biophysica Acta·Mary C HuntStefan E H Alexson
Apr 17, 2003·FEMS Microbiology Reviews·J Kalervo HiltunenAner Gurvitz
Oct 20, 2006·Biochimica Et Biophysica Acta·Ida J van der Klei, Marten Veenhuis
Oct 10, 2006·Biochimica Et Biophysica Acta·Yves PoirierJ Kalervo Hiltunen
Sep 5, 2006·Biochimica Et Biophysica Acta·Aner Gurvitz, Hanspeter Rottensteiner

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