In Silico Analyses of Rice Thionin Genes and the Antimicrobial Activity of OsTHION15 Against Phytopathogens

Phytopathology
Krissana BoonpaSupaart Sirikantaramas

Abstract

Thionins are a family of antimicrobial peptides. We performed in silico expression analyses of the 44 rice (Oryza sativa) thionins (OsTHIONs). Modulated expression levels of OsTHIONs under different treatments suggest their involvement in many processes, including biotic, abiotic, and nutritional stress responses, and in hormone signaling. OsTHION15 (LOC_Os06g32600) was selected for further characterization based on several in silico analyses. OsTHION15 in O. sativa subsp. indica 'KDML 105' was expressed in all of the tissues and organs examined, including germinating seed, leaves, and roots of seedlings and mature plants, and inflorescences. To investigate the antimicrobial activity of OsTHION15, we produced a recombinant peptide in Escherichia coli Rosetta-gami (DE3). The recombinant OsTHION15 exhibited inhibitory activities toward rice-pathogenic bacteria such as Xanthomonas oryzae pv. oryzae and Pectobacterium carotovorum pv. atroseptica, with minimum inhibitory concentrations of 112.6 and 14.1 µg ml-1, respectively. A significant hyphal growth inhibition was also observed toward Fusarium oxysporum f. sp. cubense and Helminthosporium oryzae. In addition, we demonstrated the in planta antibacterial activity of this peptide i...Continue Reading

References

May 1, 1981·European Journal of Biochemistry·L CarrascoF García-Olmedo
May 1, 1995·Molecular Plant-microbe Interactions : MPMI·K M KraghJ D Mikkelsen
Jan 15, 1997·European Journal of Biochemistry·A RomeroF García-Olmedo
Dec 3, 1999·Applied and Environmental Microbiology·K ThevissenW F Broekaert
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·R E Hancock, M G Scott
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PearceC A Ryan
Feb 21, 2002·Biochimica Et Biophysica Acta·Alexandre CoulonAndre Lopez
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
May 23, 2006·Cellular and Molecular Life Sciences : CMLS·B Stec
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Kevin A T SilversteinKathryn A VandenBosch
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jul 25, 2009·Plant Biotechnology Journal·Frank SainsburyGeorge P Lomonossoff
Jan 1, 2008·Advances in Bioinformatics·Tomas HruzPhilip Zimmermann
Sep 1, 2006·Molecular Plant Pathology·David O Niño-LiuAdam J Bogdanove
Oct 16, 2010·Peptides·Dan S Bolintineanu, Yiannis N Kaznessis
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Nov 24, 2011·Nucleic Acids Research·David M GoodsteinDaniel S Rokhsar
Sep 19, 2012·The New Phytologist·Tina KyndtGodelieve Gheysen
Mar 13, 2013·Plant Molecular Biology·Hadrien Peyret, George P Lomonossoff
Jul 1, 2008·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Y M ShabanaY M Rashad
Aug 28, 2014·Frontiers in Plant Science·Eva MiedesAntonio Molina
Feb 11, 2015·The New Phytologist·Anna PiaseckaPaweł Bednarek
Feb 14, 2015·Molecular Plant Pathology·Hongli JiTina Kyndt
Mar 18, 2016·Journal of Experimental Botany·Alice MacQueen, Joy Bergelson
May 23, 2017·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Lin XiaZhang Zhang

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