In silico analysis identifies a putative cell-of-origin for BRAF fusion-positive cerebellar pilocytic astrocytoma

PloS One
Subhi Talal Younes, Betty Herrington

Abstract

Childhood cancers are increasingly recognized as disorders of cellular development. This study sought to identify the cellular and developmental origins of cerebellar pilocytic astrocytoma, the most common brain tumor of childhood. Using publicly available gene expression data from pilocytic astrocytoma tumors and controlling for driver mutation, a set of developmental-related genes which were overexpressed in cerebellar pilocytic astrocytoma was identified. These genes were then mapped onto several developmental atlases in order to identify normal cells with similar gene expression patterns and the developmental trajectory of those cells was interrogated. Eight known neuro-developmental genes were identified as being expressed in cerebellar pilocytic astrocytoma. Mapping those genes or their orthologs onto mouse neuro-developmental atlases identified overlap in their expression within the ventricular zone of the cerebellar anlage. Further analysis with a single cell RNA-sequencing atlas of the developing mouse cerebellum defined this overlap as occurring in ventricular zone progenitor cells at the division point between GABA-ergic neuronal and glial lineages, a developmental trajectory which closely mirrors that previously des...Continue Reading

References

Oct 13, 2006·The New England Journal of Medicine·Kevin C OeffingerUNKNOWN Childhood Cancer Survivor Study
Oct 28, 2008·Journal of Child Neurology·Roger J Packer
Sep 9, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul A NorthcottMichael D Taylor
Dec 15, 2010·Nature·Paul GibsonRichard J Gilbertson
Dec 24, 2010·Neuro-oncology·Gregory T ArmstrongE Brannon Morris
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michelle MonjePhilip A Beachy
Sep 3, 2011·The Journal of Molecular Diagnostics : JMD·Yongji TianKeith L Ligon
Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·David T W JonesStefan M Pfister
Apr 16, 2013·Nature Genetics·Jinghui ZhangUNKNOWN St. Jude Children’s Research Hospital–Washington University Pediatric Cancer Genome Project
Jan 28, 2016·Nature·Charles Y LinPaul A Northcott
Apr 5, 2016·Acta Neuropathologica·Vijay RamaswamyScott L Pomeroy
Aug 21, 2019·Nature Communications·Zachary J ReitmanRameen Beroukhim

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Software Mentioned

R package limma
topGO
Cell Seek
Cell
R
Monocle
BrainSpan
maEndtoEnd
scApeX
GIMP

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