In Silico Analysis of HA2/Mx Chimera Peptide for Developing an Adjuvanted Vaccine to Induce Immune Responses Against Influenza Viruses

Advanced Pharmaceutical Bulletin
Sina SoleimaniMohsen Lotfi

Abstract

The direct transmission of avian influenza viruses to human and increasing drug resisted strains posing new threats for public health. Therefore, development of efficient vaccines is needed to generate protective and persistent immunity to the viruses. Three motifs of Mx protein sequence in human, mouse and poultry located in interferon induced (GTP ase) domain were candidate as biologic adjuvant for enhancing the immune responses against influenza virus. Chimera proteins composed with the conserved HA2 subunit of influenza virus and the Mx motifs named HA2/Mx were modeled and evaluated by in silico analysis includes bioinformatics algorithms in order to explore biological characteristics of these peptides. Amongst the predicted models, HA2/Mx1 peptide showed the better results following protein structures prediction, antigenic epitopes determination and model quality evaluation. Comparative homology modeling was performed with Swiss Model and the model was validated using ProSA. Epitope predictions revealed the construct could induce both B and T cell epitopes that expect a high immune response. Taken together, these data indicate that the HA2/Mx1 chimera peptide can be potentiated for developing an adjuvant-fused influenza va...Continue Reading

References

Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y P LinA Hay
Nov 5, 2005·Cell·Charles J Russell, Robert G Webster
Nov 8, 2005·Journal of Clinical Microbiology·K M ButtYi Guan
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Apr 3, 2007·Trends in Microbiology·David J Vigerust, Virginia L Shepherd
Nov 24, 2007·Expert Review of Vaccines·Tamar Ben-Yedidia, Ruth Arnon
Dec 8, 2007·Microbes and Infection·Otto HallerGeorg Kochs
Jul 30, 2008·Microbes and Infection·Kristie M GrebeJack R Bennink
Jun 12, 2010·The Journal of Biological Chemistry·Steven J Gamblin, John J Skehel
Aug 21, 2010·Immunology·Namrata Tomar, Rajat K De
Jun 23, 2011·BMC Bioinformatics·Yulong WangParantu K Shah
Sep 13, 2011·Research in Veterinary Science·Shahla ShahsavandiMohammad Majid Ebrahimi
Oct 5, 2011·Virus Research·S M KangR W Compans
Jan 19, 2013·Annual Review of Medicine·Natalie Pica, Peter Palese
May 31, 2013·Frontiers in Immunology·Sunita AwateGeorge Mutwiri
Sep 24, 2013·Expert Review of Vaccines·Orli Even-OrYechezkel Barenholz
Jan 16, 2014·Clinical and Experimental Vaccine Research·Young-Tae LeeSang-Moo Kang
Apr 23, 2014·Annals of Indian Academy of Neurology·S Vinoth Kanna, K Bhanu
May 3, 2014·Viruses·Han ZhangBao-Zhong Wang

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Citations

Dec 6, 2020·BMC Molecular and Cell Biology·Davod JafariMohammad Morad Farajollahi

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