Apr 3, 2020

In silico approach for designing of a multi-epitope based vaccine against novel Coronavirus (SARS-COV-2)

BioRxiv : the Preprint Server for Biology
R. Saha, Burra VLS Prasad

Abstract

A novel Coronavirus (SARS-COV-2) has now become a global pandemic. Considering the severity of infection and the associated mortalities, there is an urgent need to develop an effective preventive measure against this virus. In this study, we have designed a novel vaccine construct using computational strategies. Spike (S) glycoprotein is the major antigenic component that trigger the host immune responses. Detailed investigation of S protein with various immunoinformatics tools enabled us to identify 5 MHC I and 5 MHC II B-cell derived T-cell epitopes with VaxiJen score > 1 and IC50 value < 100nM. These epitopes were joined with a suitable adjuvant and appropriate linkers to form a multi-epitope based vaccine construct. Further, in silico testing of the vaccine construct for its antigenicity, allergenicity, solubility, and other physicochemical properties showed it to be safe and immunogenic. Suitable tertiary structure of the vaccine protein was generated using 3Dpro of SCRATCH suite, refined with GalaxyRefine, and validated with ProSA, PROCHECK, and ERRAT server. Finally, molecular docking studies were performed to ensure a favorable binding affinity between the vaccine construct and TLR3 receptor. The designed multi-epitope ...Continue Reading

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Mentioned in this Paper

Gene Polymorphism
Genome-Wide Association Study
Short Tandem Repeat
Quantitative Trait Loci
Cellular Process
Genome
Gene Expression
Genomics
Estring
Comparative Genomic Analysis

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