Abstract
Genetically modified (GM) crops require allergenicity and toxicity assessment of the novel protein(s) to ensure complete safety to the consumers. These assessments are performed in accordance with the guidelines proposed by Codex (2003) and ICMR (2008). The guidelines recommend sequence homology analysis as a preliminary step towards allergenicity prediction, later in vitro experiments may be performed to confirm allergenicity. In the present study, an in silico approach is employed to evaluate the allergenic potential of six transgenes routinely used for the development of GM food crops. Among the genes studied, manganese superoxide dismutase (MnSOD) and osmotin shares greater than 90% identity with Hev b 10 and Cap a 1w, respectively. Chitinase shares greater than 70% identity with allergens namely Pers a 1 and Hev b 11, and fungal chitinase showed significant IgE binding with 7 of 75 patients' sera positive to different food extracts. Glucanases (alfalfa, wheat) and glycine betaine aldehyde dehydrogenase gene share 50% homology with allergens like - Ole e 9, Cla h 10 and Alt a 10. The results demonstrate the allergenic potential of six genes and can serve as a guide for selection of transgenes to develop GM crops.
References
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Henikoff, J G Henikoff
Nov 1, 1990·Allergy·B WüthrichS G Johansson
Jul 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H AleniusT Palosuo
Jan 1, 1996·Critical Reviews in Food Science and Nutrition·D D MetcalfeR L Fuchs
Apr 3, 1998·The Journal of Allergy and Clinical Immunology·T YagamiZ Ikezawa
Jun 24, 1998·Allergy·J C García OrtizJ Bellido
Oct 17, 1998·The Journal of Biological Chemistry·S SowkaH Breiteneder
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·C BlancoG Salcedo
Dec 5, 2000·Current Opinion in Immunology·R M Helm, A W Burks
Sep 10, 2002·International Archives of Allergy and Immunology·Ronald E HilemanSusan L Hefle
Nov 9, 2002·Bioinformatics·J T Reese, W R Pearson
Jan 10, 2003·Nucleic Acids Research·Ovidiu IvanciucWerner Braun
Dec 6, 2003·The Journal of Allergy and Clinical Immunology·Scott H SichererHugh A Sampson
May 7, 2004·European Journal of Biochemistry·Anna AnderssonAnnika Scheynius
May 8, 2004·The Journal of Allergy and Clinical Immunology·Hugh A Sampson
Mar 4, 2006·Allergy·A K SinghN Arora
May 4, 2006·Current Opinion in Allergy and Clinical Immunology·Lynette Pei-Chi Shek, Bee Wah Lee
Feb 6, 2007·Immunology and Allergy Clinics of North America·Catherine H ScheinWerner Braun
Nov 28, 2008·Journal of Agricultural and Food Chemistry·Abinav K SinghNaveen Arora
Nov 1, 2005·Expert Review of Clinical Immunology·Richard E Goodman, Sue L Hefle
Aug 16, 2011·Journal of Agricultural and Food Chemistry·Prerna SharmaNaveen Arora
Citations
Feb 6, 2015·Journal of the Science of Food and Agriculture·Ankita MishraNaveen Arora
Sep 1, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ankita MishraNaveen Arora
Apr 19, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Davi Felipe FariasAna Fontenele Urano Carvalho
Dec 4, 2014·Molecular Immunology·Prerna SharmaNaveen Arora
Sep 1, 2017·International Journal of Systematic and Evolutionary Microbiology·Bilguun OyuntsetsegSeung Bum Kim
Feb 28, 2014·ISRN Microbiology·Rogério SchünemannLidia Mariana Fiuza
Jul 3, 2021·Foods·Ankita Mishra, Ashok Kumar