In Silico Characterization and Structural Modeling of Dermacentor andersoni p36 Immunosuppressive Protein

Advances in Bioinformatics
Martin Omulindi OyugiNaftaly Githaka

Abstract

Ticks cause approximately $17-19 billion economic losses to the livestock industry globally. Development of recombinant antitick vaccine is greatly hindered by insufficient knowledge and understanding of proteins expressed by ticks. Ticks secrete immunosuppressant proteins that modulate the host's immune system during blood feeding; these molecules could be a target for antivector vaccine development. Recombinant p36, a 36 kDa immunosuppressor from the saliva of female Dermacentor andersoni, suppresses T-lymphocytes proliferation in vitro. To identify potential unique structural and dynamic properties responsible for the immunosuppressive function of p36 proteins, this study utilized bioinformatic tool to characterize and model structure of D. andersoni p36 protein. Evaluation of p36 protein family as suitable vaccine antigens predicted a p36 homolog in Rhipicephalus appendiculatus, the tick vector of East Coast fever, with an antigenicity score of 0.7701 that compares well with that of Bm86 (0.7681), the protein antigen that constitute commercial tick vaccine Tickgard™. Ab initio modeling of the D. andersoni p36 protein yielded a 3D structure that predicted conserved antigenic region, which has potential of binding immunomodul...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 1996·Protein Science : a Publication of the Protein Society·R A LaskowskiJ M Thornton
Jan 1, 1997·Methods in Enzymology·D EisenbergJ U Bowie
Jan 17, 2002·The Journal of Biological Chemistry·Gérard LeboulleEdmond Godfroid
May 23, 2002·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Lenore CowenBonnie Berger
Oct 24, 2002·International Journal for Parasitology·Vishvanath NeneRichard Bishop
Jun 7, 2003·Parasite Immunology·Francisco J Alarcon-ChaidezStephen Wikel
Sep 4, 2003·Genome Research·Li LiDavid S Roos
Oct 12, 2004·Insect Biochemistry and Molecular Biology·Vishvanath NeneRichard Bishop
Jun 9, 2005·Parasitology·F Jongejan, G Uilenberg
Oct 10, 2007·Veterinary Research Communications·J de la FuenteE F Blouin
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Jose de la FuenteDaniel E Sonenshine
Nov 18, 2009·Cell Host & Microbe·Jianfeng DaiErol Fikrig
Feb 4, 2010·Archives of Microbiology·Roy D Sleator, Paul Walsh
Sep 1, 2011·Nature Reviews. Genetics·Diethard Tautz, Tomislav Domazet-Lošo
Sep 9, 2011·Current Protocols in Bioinformatics·Steve FischerChristian J Stoeckert
Jul 7, 2012·The Veterinary Journal·Luís F PariziItabajara da Silva Vaz
Jan 8, 2013·Vaccine·Juan A Moreno-CidUNKNOWN SUB/AKR Vaccine Study Group
Jun 29, 2013·Current Opinion in Virology·Daniel W Kulp, William R Schief
Jul 13, 2013·Frontiers in Cellular and Infection Microbiology·Octavio MerinoJosé de la Fuente
Apr 9, 2014·Veterinary Parasitology·Rao Z AbbasZafar Iqbal
Oct 22, 2014·The British Journal of Nutrition·Monika PaaselaJarno Honkanen
Aug 21, 2015·Expert Review of Vaccines·José de la Fuente, Marinela Contreras
Feb 3, 2016·Trends in Parasitology·Jindřich ChmelařMichail Kotsyfakis
Dec 3, 2016·PLoS Neglected Tropical Diseases·Daiane P OldigesCarlos E Suarez
Feb 9, 2017·Ticks and Tick-borne Diseases·Carolina K RangelItabajara da Silva Vaz

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Citations

Mar 27, 2020·Frontiers in Cellular and Infection Microbiology·Fangfang WangJinlin Zhou

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Methods Mentioned

BETA
NMR

Software Mentioned

Scratch
Sprint
Coach
Blastp
OrthoMCL
Verify 3D
QUARK
ModRefiner
Clustal Omega
SVMTrip

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