In-Silico Characterization of Glycosyl Hydrolase Family 1 β-Glucosidase from Trichoderma asperellum UPM1.

International Journal of Molecular Sciences
Mohamad Farhan Mohamad SobriNorhayati Ramli

Abstract

β-glucosidases (Bgl) are widely utilized for releasing non-reducing terminal glucosyl residues. Nevertheless, feedback inhibition by glucose end product has limited its application. A noticeable exception has been found for β-glucosidases of the glycoside hydrolase (GH) family 1, which exhibit tolerance and even stimulation by glucose. In this study, using local isolate Trichoderma asperellum UPM1, the gene encoding β-glucosidase from GH family 1, hereafter designated as TaBgl2, was isolated and characterized via in-silico analyses. A comparison of enzyme activity was subsequently made by heterologous expression in Escherichia coli BL21(DE3). The presence of N-terminal signature, cis-peptide bonds, conserved active site motifs, non-proline cis peptide bonds, substrate binding, and a lone conserved stabilizing tryptophan (W) residue confirms the identity of Trichoderma sp. GH family 1 β-glucosidase isolated. Glucose tolerance was suggested by the presence of 14 of 22 known consensus residues, along with corresponding residues L167 and P172, crucial in the retention of the active site's narrow cavity. Retention of 40% of relative hydrolytic activity on ρ-nitrophenyl-β-D-glucopyranoside (ρNPG) in a concentration of 0.2 M glucose w...Continue Reading

References

May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 1, 1995·Computer Applications in the Biosciences : CABIOS·M G Claros
Jan 1, 1996·Methods in Enzymology·J GarnierB Robson
Feb 5, 1999·Journal of Molecular Biology·A JabsR Hilgenfeld
Aug 15, 2001·European Journal of Biochemistry·P VäljamäeG Johansson
Aug 30, 2002·Applied and Environmental Microbiology·Markku SaloheimoMerja Penttilä
Sep 17, 2002·Briefings in Bioinformatics·Christian J A SigristPhilipp Bucher
Sep 24, 2004·Science in China. Series C, Life Sciences·Zhao YueGao Peiji
May 11, 2006·Biotechnology and Bioengineering·Y-H Percival Zhang, Lee R Lynd
May 8, 2007·Nucleic Acids Research·Lukas KällErik L L Sonnhammer
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
Feb 12, 2008·Current Protocols in Molecular Biology·S Watkins
May 13, 2010·Applied Microbiology and Biotechnology·André Schuster, Monika Schmoll
May 14, 2010·Current Biology : CB·Yariv BrotmanAda Viterbo
Oct 19, 2012·Protein Engineering, Design & Selection : PEDS·Hsiao-Lin LeePo-Huang Liang
Dec 20, 2012·Bioinformation·Nathan Vinod KumarJayavel Sridhar
Feb 28, 2013·BMC Research Notes·Ulrike SchumannMing-Bo Wang
Aug 29, 2013·BioMed Research International·Pragya TiwariNeelam S Sangwan
Feb 18, 2014·Journal of Basic Microbiology·Judyta StrakowskaJerzy Chełkowski
Apr 15, 2014·Journal of Agricultural and Food Chemistry·Chia-wen C HsiehLisbeth G Thygesen
Jun 11, 2014·Acta Crystallographica. Section D, Biological Crystallography·Priscila Oliveira de GiuseppeMario Tyago Murakami
Jan 1, 2013·Biomolecules·Annette SørensenBirgitte K Ahring
Jan 13, 2015·Evolutionary Bioinformatics Online·Muhammad Tariq PervezMuhammad Shoaib
Feb 24, 2015·Journal of Biotechnology·Lukasz WieteskaDominik Gront
Nov 26, 2015·Scientific Reports·Yang YangYazhong Xiao
Jan 8, 2016·Evolutionary Bioinformatics Online·Kirsten E BeattieJohn B O Mitchell
Feb 6, 2016·Applied Microbiology and Biotechnology·Boyang GuoKouichi Nozaki
May 24, 2016·Trends in Biochemical Sciences·Vijai K GuptaThaddeus Ezeji
Sep 8, 2016·Acta Crystallographica. Section D, Structural Biology·Maria Rutkiewicz-KrotewiczAnna Bujacz
Feb 13, 2017·Journal of Structural Biology·Panjiao PangZhong Wang
Oct 17, 2017·Bioinformatics·José Juan Almagro ArmenterosOle Winther
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Feb 20, 2019·Nature Biotechnology·José Juan Almagro ArmenterosHenrik Nielsen
Mar 1, 2019·Protein and Peptide Letters·Dilani G GamageTimothy G Russell

❮ Previous
Next ❯

Citations

Apr 4, 2021·International Journal of Molecular Sciences·Hongfei SuFen Wei

❮ Previous
Next ❯

Datasets Mentioned

BETA
KX772748.1
ARW78142.1

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ProtScale
MitoProt
Molecular Evolutionary Genetics Analysis ( MEGA ) X
DeepLoc
BLASTp
BLAST
MODEL
BioEdit Sequence Alignment Editor
PROSITE
ExPASY

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.