In silico characterization of microRNAs-like sequences in the genome of Paracoccidioides brasiliensis

Genetics and Molecular Biology
Juliana S de CurcioCélia Maria de Almeida Soares

Abstract

Eukaryotic cells have different mechanisms of post-transcriptional regulation. Among these mechanisms, microRNAs promote regulation of targets by cleavage or degradation of the mRNA. Fungi of the Paracoccidioides complex are the etiological agents of the main systemic mycosis of Latin America. These fungi present a plasticity to adapt and survive in different conditions, and the presence of microRNAs-like molecules could be part of the mechanisms that provide such plasticity. MicroRNAs produced by the host influence the progression of this mycosis in the lungs besides regulating targets involved in apoptosis in macrophage, activation of T and B cells and the production of cytokines. Therefore, this work analyzed the presence of regions in the genome of this fungus with a potential to encode microRNAs-like molecules. Here we show by analysis of sequence similarity the presence of 18 regions, putatively coding for microRNAs-like molecules in the Paracoccidioides brasiliensis genome. We also described the conservation of dicer and argonaut proteins and the cognate transcripts induced in the yeast parasitic phase. This work represents a starting point for the analysis of the presence of those molecules in the morphological stages o...Continue Reading

References

Apr 25, 2003·Nature·James E GalaganBruce Birren
Nov 15, 2003·Nature·Andreas LingelMichael Sattler
Nov 15, 2003·Nature·Kelley S YanMing-Ming Zhou
Jul 10, 2004·Cell·Haidi ZhangWitold Filipowicz
Jul 31, 2004·Science·Ji-Joon SongLeemor Joshua-Tor
Apr 26, 2005·The Journal of Biological Chemistry·Maria Sueli S FelipeUNKNOWN PbGenome Network
Sep 3, 2005·FEBS Letters·Hitoshi Nakayashiki
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Huiling HeAlbert de la Chapelle
Jan 6, 2006·Cellular and Molecular Life Sciences : CMLS·B H ZhangT A Anderson
Jun 21, 2006·Journal of Molecular Evolution·Hitoshi NakayashikiShigeyuki Mayama
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·L Joshua-Tor
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Feb 12, 2008·Current Protocols in Molecular Biology·Angie L BookoutMartha F Kramer
Sep 12, 2009·Science·Ines A DrinnenbergDavid P Bartel
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Jun 11, 2010·Indian Journal of Ophthalmology·Sujata DasAparajita Mallick
Nov 12, 2010·Fungal Genetics and Biology : FG & B·Guilhem JanbonYong-Sun Bahn
Mar 31, 2011·Database : the Journal of Biological Databases and Curation·Michele Magrane, Uniprot Consortium
Jun 28, 2011·BMC Biotechnology·Ingrid BalcellsPeter K Busk
Sep 17, 2011·Journal of Proteomics·Tereza C V RezendeCélia M A Soares
Sep 20, 2011·Nature Structural & Molecular Biology·Akihisa TsutsumiYukihide Tomari
Nov 26, 2011·Algorithms for Molecular Biology : AMB·Ronny LorenzIvo L Hofacker
Aug 27, 2014·PloS One·Mirelle Garcia Silva-BailãoCélia Maria de Almeida Soares
Jan 15, 2015·Scientific Reports·Roberta Peres da SilvaLysangela R Alves
Jul 5, 2015·Database : the Journal of Biological Databases and Curation·Andrew NdhlovuScott Hazelhurst
Dec 18, 2015·Nucleic Acids Research·Robert D FinnAlex Bateman
Apr 12, 2016·Methods : a Companion to Methods in Enzymology·Ronny LorenzIvo L Hofacker
Jul 31, 2016·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T L CrostonB J Green

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Citations

Jul 16, 2019·PLoS Neglected Tropical Diseases·David Aciole BarbosaLuiz R Nunes
Nov 7, 2020·Microbes and Infection·Juliana S de CurcioCélia Maria de Almeida Soares
Jul 16, 2021·Bioinformatics and Biology Insights·Md Irtija AhsanSyed Sayeem Uddin Ahmed

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Methods Mentioned

BETA
PCR

Software Mentioned

Fasta
TreeView
Perl
BLASTp
Blastn
InrsectBED
RNAfold
CLUSTALX2
BEDTools

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