Oct 31, 2017

In silico CRISPR-based sgRNA design

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Yuanli WangQi Liu

Abstract

CRISPR-based genome editing has been widely implemented in various cell types. In-silico single guide RNA (sgRNA) design is a key step for successful gene editing using CRISPR system. Continuing efforts are made to refine in-silico sgRNA design with high on-target efficacy and reduced off-target effects. In this paper, we summarize the present sgRNA design tools, and show that efficient in-silico models can be built that integrate current heterogeneous genome-editing data to derive unbiased sgRNA design rules and identify key features for improving sgRNA design. Our review shows that systematic comparisons and evaluation of on-target and off-target effects of sgRNA will allow more precise genome editing and gene therapies using the CRISPR system.

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Mentioned in this Paper

In Silico
Genes
CRISPR-Cas Systems
Gene Editing
Evaluation
Cell Type
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR Spacers
RNA, Guide

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