In silico, in vitro and in vivo analyses of dipeptidyl peptidase IV inhibitory activity and the antidiabetic effect of sodium caseinate hydrolysate

Food & Function
Cheng-Hong HsiehKuo-Chiang Hsu

Abstract

The frequency (A), a novel in silico parameter, was developed by calculating the ratio of the number of truncated peptides with Xaa-proline and Xaa-alanine to all peptide fragments from a protein hydrolyzed with a specific protease. The highest in vitro DPP-IV inhibitory activity (72.7%) was observed in the hydrolysate of sodium caseinate by bromelain (Cas/BRO), and the constituent proteins of bovine casein also had relatively high A values (0.10-0.17) with BRO hydrolysis. 1CBR (the <1 kDa fraction of Cas/BRO) showed the greatest in vitro DPP-IV inhibitory activity of 77.5% and was used for in vivo test by high-fat diet-fed and low-dose streptozotocin-induced diabetic rats. The daily administration of 1CBR for 6 weeks was effective to improve glycaemic control in diabetic rats. The results indicate that the novel in silico method has the potential as a screening tool to predict dietary proteins to generate DPP-IV inhibitory and antidiabetic peptides.

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Citations

Mar 16, 2017·Food Research International·Alice B NongoniermaRichard J FitzGerald
Jun 7, 2020·Applied Biochemistry and Biotechnology·Andréia Monique LermenDaniel Joner Daroit
Jan 27, 2019·International Journal of Molecular Sciences·Rui LiuHao Wu
Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing GaoXueying Mao
Oct 23, 2019·International Journal of Molecular Sciences·Honey Lyn R GomezYu-Wei Chang

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