In silico, in vitro and in vivo analysis of putative virulence factors identified in large clostridial toxin-negative, binary toxin producing C. difficile strains

Anaerobe
Grace O AndrogaThomas V Riley

Abstract

The relevance of large clostridial toxin-negative, binary toxin-producing (A-B-CDT+) Clostridium difficile strains in human infection is still controversial. In this study, we investigated putative virulence traits that may contribute to the role of A-B-CDT+C. difficile strains in idiopathic diarrhea. Phenotypic assays were conducted on 148 strains of C. difficile comprising 10 different A-B-CDT+C. difficile ribotypes (RTs): 033, 238, 239, 288, 585, 586, QX143, QX444, QX521 and QX629. A subset of these isolates (n = 53) was whole-genome sequenced to identify genetic loci associated with virulence and survival. Motility studies showed that with the exception of RT 239 all RTs tested were non-motile. C. difficile RTs 033 and 288 had deletions in the F2 and F3 regions of their flagella operon while the F2 region was absent from strains of RTs 238, 585, 586, QX143, QX444, QX521 and QX629. The flagellin and flagella cap genes, fliC and fliD, respectively, involved in adherence and host colonization, were conserved in all strains, including reference strains. All A-B-CDT+C. difficile strains produced at least three extracellular enzymes (deoxyribonuclease, esterase and mucinase) indicating that these are important extracellular prote...Continue Reading

References

Mar 9, 1978·The New England Journal of Medicine·J G BartlettA B Onderdonk
May 1, 1976·Journal of Medical Microbiology·S Hafiz, C L Oakley
May 1, 1992·Journal of Medical Microbiology·S V Seddon, S P Borriello
Oct 1, 1990·Journal of Clinical Microbiology·M DelméeG Burtonboy
Jan 1, 1990·Reviews of Infectious Diseases·S P BorrielloS Seddon
Sep 1, 1988·Infection and Immunity·M R PopoffP Boquet
Apr 1, 1987·Journal of Clinical Pathology·T V Riley
Feb 1, 1987·Journal of Clinical Microbiology·P J SwiatekC H Lee
Aug 1, 1981·Journal of Clinical Microbiology·E K Steffen, D J Hentges
Jan 6, 2000·Postgraduate Medical Journal·J D Urschel
Jun 16, 2004·FEMS Immunology and Medical Microbiology·Denise DrudyCiarán P Kelly
Jun 25, 2005·Bioinformatics·Tim J CarverJulian Parkhill
Feb 1, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Frédéric BarbutJean-Claude Petit
Jun 17, 2009·Nature Reviews. Microbiology·Maja RupnikDale N Gerding
Jun 15, 2010·Diagnostic Microbiology and Infectious Disease·Simon D GoldenbergGary L French
Jul 14, 2011·Emerging Infectious Diseases·Sabrina BacciKatharina E P Olsen
Jul 1, 2010·Toxins·Xingmin SunHanping Feng
Aug 2, 2012·Infection and Immunity·Annie AubrySusan M Logan
Oct 23, 2012·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·David B StewartJohn Hegarty
May 15, 2013·International Journal of Antimicrobial Agents·Pierre TattevinChristian Michelet
Aug 13, 2013·The Journal of Infectious Diseases·Sarah A KuehneNigel P Minton
Jan 1, 2014·FEMS Microbiology Letters·Melanie L HuttonDena Lyras
May 13, 2014·Trends in Microbiology·Daniel Paredes-SabjaJoseph A Sorg
Feb 11, 2015·Trends in Microbiology·Emma StevensonSarah A Kuehne
Jul 16, 2016·PLoS Pathogens·Shelley A LyonDena Lyras
Sep 9, 2017·Cleveland Clinic Journal of Medicine·Arjun Srinivasan
Feb 6, 2018·Annals of Laboratory Medicine·Nobuaki Mori, Takashi Takahashi
Feb 11, 2018·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A DurovicS Tschudin-Sutter
Nov 1, 2018·The New England Journal of Medicine·Shelley S MagillUNKNOWN Emerging Infections Program Hospital Prevalence Survey Team

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