In silico Prediction of Protein-Protein Interaction Network Induced by Manganese II in Meyerozyma guilliermondii

Frontiers in Microbiology
France Anne Dias Ruas, Renata Guerra-Sá

Abstract

Recently, there has been an increasing interest in the use of yeast to produce biosorbent materials, because yeast is economical to use, adaptable to a variety of conditions, and amenable to morphological manipulations to yield better raw biomaterials. Previous studies from our laboratory have shown that Meyerozyma guilliermondii, a non-pathogenic haploid yeast (ascomycete), exhibits excellent biosorption capacity for Mn2+, as demonstrated by kinetic analyses. Shotgun/bottom-up analyses of soluble fractions revealed a total of 1257 identified molecules, with 117 proteins expressed in the absence of Mn2+ and 69 expressed only in the presence of Mn2+. In this article, we describe the first in silico prediction and screening of protein-protein interactions (PPIs) in M. guilliermondii using experimental data from shotgun/bottom-up analyses. We also present the categorization of biological processes (BPs), molecular functions (MFs), and metabolic pathways of 71 proteins upregulated in the M. guilliermondii proteome in response to stress caused by an excess of Mn2+ ions. Most of the annotated proteins were related to oxidation-reduction processes, metabolism, and response to oxidative stress. We identified seven functional enrichment...Continue Reading

References

Jun 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C Thorpe, J J Kim
May 1, 1995·Biotechnology Progress·B Volesky, Z R Holan
Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
Dec 10, 1998·Nucleic Acids Research·H OgataM Kanehisa
Jun 24, 2000·Nature·A Pandey, M Mann
Dec 2, 2000·Nature Biotechnology·B SchwikowskiS Fields
Mar 16, 2002·Current Topics in Medicinal Chemistry·N ErcalN Aykin-Burns
Mar 30, 2002·Critical Reviews in Oncology/hematology·Fei Chen, Xianglin Shi
May 27, 2004·Microbiological Research·Pawel KaszyckiHenryk Koloczek
Feb 1, 2005·The Science of the Total Environment·D Barrie Johnson, Kevin B Hallberg
May 28, 2005·Bioinformatics·Jason McDermottRam Samudrala
Aug 30, 2006·Genome Biology·Johannes Goll, Peter Uetz
Oct 13, 2006·Biochimica Et Biophysica Acta·Michael Schrader, H Dariush Fahimi
Nov 1, 2008·Current Opinion in Microbiology·Ellen M Fox, Barbara J Howlett
Mar 25, 2009·FEBS Letters·Chanchal Kumar, Matthias Mann
May 22, 2009·BioFactors·Nina A BonekampMichael Schrader
May 26, 2009·Nature·Geraldine ButlerChristina A Cuomo
Jun 9, 2009·Free Radical Biology & Medicine·Etsuo Niki
Mar 2, 2010·The Journal of Experimental Biology·R N M Sehgal
Apr 9, 2010·FEMS Microbiology Reviews·Robert Wysocki, Markus J Tamás
Jul 14, 2010·Nature Biotechnology·Parag Mallick, Bernhard Kuster
May 10, 2011·Biochimica Et Biophysica Acta·Douglas B MurrayMasaru Tomita
May 1, 2005·Environmental Toxicology and Pharmacology·Keith M EriksonMichael Aschner
Mar 23, 2012·Antioxidants & Redox Signaling·Shradha PrabhulkarChen-Zhong Li
Jun 8, 2012·International Journal of Cell Biology·Katarína Smolková, Petr Ježek
Oct 19, 2013·Bioresource Technology·Akhilesh Kumar ChaurasiaShree Kumar Apte
Jan 15, 2014·PloS One·Dagmar HosinerEdda Klipp
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob
Jan 13, 2015·Expert Review of Proteomics·Shachuan FengEdouard C Nice
Dec 23, 2015·International Journal of Molecular Sciences·Arif Tasleem JanQazi Mohd Rizwanul Haq
Sep 18, 2016·World Journal of Microbiology & Biotechnology·Christopher Chibueze AzubuikeGideon Chijioke Okpokwasili
Dec 3, 2016·Nucleic Acids Research·Shujiro OkudaYasushi Ishihama
Jun 3, 2017·Current Opinion in Structural Biology·T M CafarelliM Vidal
Aug 2, 2017·Journal of Biochemical and Molecular Toxicology·M Serhat ÖzaslanMehmet Çiftci
Aug 30, 2017·Environmental Science and Pollution Research International·Raheleh KafaeiBahman Ramavandi
Nov 8, 2017·Bioinformatics·Lauro Ângelo Gonçalves de MoraesLeandro Marcio Moreira
Nov 11, 2018·Journal of Proteomics·France Anne Dias RuasRenata Guerra-Sá

❮ Previous
Next ❯

Citations

Oct 24, 2020·Current Genomics·Mst Shamima KhatunHiroyuki Kurata
Feb 24, 2021·World Journal of Microbiology & Biotechnology·Vijaya RaniRajeev Kaushik
Aug 10, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Anamika SaralSameer Suresh Bhagyawant

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
protein folding

Software Mentioned

Blast2GO
STRING
PSORT
Basic Local Alignment Search Tool ( BLAST )
BLASTp
TabPath
MaxQuant
PEAKS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.