In situ- Like Aerosol Inhalation Exposure for Cytotoxicity Assessment Using Airway-on-Chips Platforms

Frontiers in Bioengineering and Biotechnology
Shani Elias-KirmaJosué Sznitman

Abstract

Lung exposure to inhaled particulate matter (PM) is known to injure the airway epithelium via inflammation, a phenomenon linked to increased levels of global morbidity and mortality. To evaluate physiological outcomes following PM exposure and concurrently circumvent the use of animal experiments, in vitro approaches have typically relied on traditional assays with plates or well inserts. Yet, these manifest drawbacks including the inability to capture physiological inhalation conditions and aerosol deposition characteristics relative to in vivo human conditions. Here, we present a novel airway-on-chip exposure platform that emulates the epithelium of human bronchial airways with critical cellular barrier functions at an air-liquid interface (ALI). As a proof-of-concept for in vitro lung cytotoxicity testing, we recapitulate a well-characterized cell apoptosis pathway, induced through exposure to 2 μm airborne particles coated with αVR1 antibody that leads to significant loss in cell viability across the recapitulated airway epithelium. Notably, our in vitro inhalation assays enable simultaneous aerosol exposure across multiple airway chips integrated within a larger bronchial airway tree model, under physiological respiratory ...Continue Reading

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Citations

Mar 5, 2020·Expert Opinion on Drug Delivery·Arbel Artzy-SchnirmanJosué Sznitman
Sep 10, 2020·Frontiers in Bioengineering and Biotechnology·Rocio J Jimenez-ValdesKambez H Benam
Nov 25, 2020·Drug Discovery Today·Carlos Victor Montefusco-PereiraClaus-Michael Lehr
Jul 20, 2021·ACS Biomaterials Science & Engineering·Kristen L HaywardBoyang Zhang
Jul 23, 2021·Advanced Healthcare Materials·Ajay Vikram SinghByung-Wook Park
Aug 1, 2021·Advanced Drug Delivery Reviews·Arbel Artzy-SchnirmanJosué Sznitman
May 12, 2021·Annual Review of Analytical Chemistry·Patarajarin AkarapipadJeong-Yeol Yoon
Oct 26, 2021·Frontiers in Bioengineering and Biotechnology·Patrick CariusClaus-Michael Lehr

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Methods Mentioned

BETA
lavages
biopsy
confocal microscopy
chip
fluorescence microscopy
Assay

Software Mentioned

Fluent
Formlabs
ImageJ

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