In situ analysis and structural elucidation of sainfoin (Onobrychis viciifolia) tannins for high-throughput germplasm screening

Journal of Agricultural and Food Chemistry
An GeaIrene Mueller-Harvey

Abstract

A rapid thiolytic degradation and cleanup procedure was developed for analyzing tannins directly in chlorophyll-containing sainfoin ( Onobrychis viciifolia ) plants. The technique proved suitable for complex tannin mixtures containing catechin, epicatechin, gallocatechin, and epigallocatechin flavan-3-ol units. The reaction time was standardized at 60 min to minimize the loss of structural information as a result of epimerization and degradation of terminal flavan-3-ol units. The results were evaluated by separate analysis of extractable and unextractable tannins, which accounted for 63.6-113.7% of the in situ plant tannins. It is of note that 70% aqueous acetone extracted tannins with a lower mean degree of polymerization (mDP) than was found for tannins analyzed in situ. Extractable tannins had between 4 and 29 lower mDP values. The method was validated by comparing results from individual and mixed sample sets. The tannin composition of different sainfoin accessions covered a range of mDP values from 16 to 83, procyanidin/prodelphinidin (PC/PD) ratios from 19.2/80.8 to 45.6/54.4, and cis/trans ratios from 74.1/25.9 to 88.0/12.0. This is the first high-throughput screening method that is suitable for analyzing condensed tanni...Continue Reading

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Citations

Apr 13, 2012·Journal of Agricultural and Food Chemistry·Martin M LorenzPeter Udén
Feb 2, 2016·Journal of Pharmaceutical and Biomedical Analysis·Honorata M RopiakIrene Mueller-Harvey
Feb 9, 2012·Phytochemistry·Elisabetta StringanoIrene Mueller-Harvey
Jul 23, 2015·International Journal for Parasitology. Drugs and Drug Resistance·Chaweewan KlongsiriwetHervé Hoste
Apr 2, 2016·Research in Veterinary Science·Andrew R WilliamsStig M Thamsborg
Mar 2, 2013·Journal of the Science of Food and Agriculture·Blasius N AzuhnwiIrene Mueller-Harvey
Aug 13, 2013·Journal of the Science of Food and Agriculture·Martin M LorenzPeter Udén
Jun 11, 2015·Journal of the Science of Food and Agriculture·Marion GirardGiuseppe Bee
Jul 6, 2011·Veterinary Parasitology·Adam NovobilskýStig Milan Thamsborg
Apr 10, 2012·Veterinary Parasitology·Anastasios SaratsisSmaragda Sotiraki
Oct 21, 2015·Journal of Agricultural and Food Chemistry·Aina RamsayIrene Mueller-Harvey
May 18, 2016·Journal of the Science of Food and Agriculture·Gong-Min LinShang-Tzen Chang
Mar 7, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kai PengYuxi Wang
Jan 21, 2018·Journal of the Science of Food and Agriculture·Chia-Yun HsuShang-Tzen Chang
Jan 7, 2020·Meat Science·Antonio NatalelloGiuseppe Luciano
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marion GirardJuha-Pekka Salminen
Feb 10, 2018·Journal of Agricultural and Food Chemistry·Jessica QuijadaIrene Mueller-Harvey
Mar 29, 2014·Journal of Agricultural and Food Chemistry·Marica T EngströmJuha-Pekka Salminen
Apr 24, 2019·Journal of Agricultural and Food Chemistry·Guillaume BillerachHélène Fulcrand

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