In situ analysis of denitrifying toluene- and m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer column.

Applied and Environmental Microbiology
A HessJ Zeyer

Abstract

A diesel fuel-contaminated aquifer was bioremediated in situ by the injection of oxidants (O2 and NO3-) and nutrients in order to stimulate microbial activity. After 3.5 years of remediation, an aquifer sample was excavated and the material was used (i) to isolate bacterial strains able to grow on selected hydrocarbons under denitrifying conditions and (ii) to construct a laboratory aquifer column in order to simulate the aerobic and denitrifying remediation processes. Five bacterial strains isolated from the aquifer sample were able to grow on toluene (strains T2 to T4, T6, and T10), and nine bacterial strains grew on toluene and m-xylene (strains M3 to M7 and M9 to M12). Strains T2 to T4, T6, and T10 were cocci, and strains M3 to M7 and M9 to M12 were rods. The morphological and physiological differences were also reflected in small sequence variabilities in domain III of the 23S rRNA and in the 16S rRNA. Comparative sequence analyses of the 16S rRNA of one isolate (T3 and M3) of each group revealed a close phylogenetic relationship for both groups of isolates to organisms of the genus Azoarcus. Two 16S rRNA-targeted oligonucleotide probes (Azo644 and Azo1251) targeting the experimental isolates, bacteria of the Azoarcus tolu...Continue Reading

References

Jan 1, 1991·Archives of Microbiology·R J SchocherJ Zeyer
Apr 1, 1991·Applied and Environmental Microbiology·P J EvansL Y Young
May 15, 1981·Journal of Molecular Biology·J BrosiusH F Noller
May 1, 1993·Applied and Environmental Microbiology·R RabusF Widdel
Aug 1, 1994·Applied and Environmental Microbiology·M R FriesJ M Tiedje
Jun 1, 1996·Applied and Environmental Microbiology·P WeissM Simon
Jun 1, 1990·Applied and Environmental Microbiology·D R Lovley, D J Lonergan

❮ Previous
Next ❯

Citations

Aug 20, 2004·Journal of Bacteriology·María J López BarragánEduardo Díaz
Jan 20, 2006·FEMS Microbiology Ecology·Patrick K JjembaJodi R Shann
Jan 15, 2008·Applied and Environmental Microbiology·Poul LarsenPer Halkjaer Nielsen
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Sep 25, 2008·FEMS Microbiology Ecology·Fernando Morgan-SagastumePer Halkjaer Nielsen
Dec 2, 2010·Environmental Microbiology·Dominik Marek DominiakPer Halkjaer Nielsen
Nov 13, 2012·Environmental Science and Pollution Research International·Asli S CigginDerin Orhon
Dec 8, 2005·Applied and Environmental Microbiology·Romy ChakrabortyJohn D Coates
Jul 26, 2005·Applied Microbiology and Biotechnology·Ralf Rabus
Feb 12, 2008·Applied and Environmental Microbiology·Kathleen TrautweinRalf Rabus
Nov 9, 2006·Biodegradation·Sabrina Botton, John R Parsons
Jan 27, 2005·Applied Microbiology and Biotechnology·Chunlong Zhang, George N Bennett
Mar 10, 2004·Applied and Environmental Microbiology·Yoshifumi ShinodaNobuo Kato
Mar 30, 2007·FEMS Microbiology Ecology·Trine Rolighed ThomsenPer Halkjaer Nielsen
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jonathan D Van HammeOwen P Ward
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Dorian MieczkowskiPiotr Świątczak
Jan 17, 2013·Environmental Microbiology·Tomonori KindaichiPer Halkjaer Nielsen
Jul 29, 2005·Current Microbiology·Barbara Morasch, Rainer U Meckenstock
Dec 2, 2017·Journal of Applied Microbiology·D M de BeerT E Cloete
Jun 19, 2001·Current Opinion in Biotechnology·F Widdel, R Rabus
Sep 5, 2009·Applied Microbiology and Biotechnology·Bin CaoKai-Chee Loh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.