In situ analysis of repair processes for oxidative DNA damage in mammalian cells

Proceedings of the National Academy of Sciences of the United States of America
Li LanAkira Yasui

Abstract

Oxidative DNA damage causes blocks and errors in transcription and replication, leading to cell death and genomic instability. Although repair mechanisms of the damage have been extensively analyzed in vitro, the actual in vivo repair processes remain largely unknown. Here, by irradiation with an UVA laser through a microscope lens, we have conditionally produced single-strand breaks and oxidative base damage at restricted nuclear regions of mammalian cells. We showed, in real time after irradiation by using antibodies and GFP-tagged proteins, rapid and ordered DNA repair processes of oxidative DNA damage in human cells. Furthermore, we characterized repair pathways by using repair-defective mammalian cells and found that DNA polymerase beta accumulated at single-strand breaks and oxidative base damage by means of its 31- and 8-kDa domains, respectively, and that XRCC1 is essential for both polymerase beta-dependent and proliferating cell nuclear antigen-dependent repair pathways of single-strand breaks. Thus, the repair of oxidative DNA damage is based on temporal and functional interactions among various proteins operating at the site of DNA damage in living cells.

References

Dec 3, 1999·Science·T Lindahl, R D Wood
Jan 29, 2000·Molecular and Cellular Biology·J G MoggsG Almouzni
Apr 25, 2000·Nucleic Acids Research·A MarintchevG P Mullen
Sep 14, 2001·Progress in Nucleic Acid Research and Molecular Biology·A E TomkinsonT A Motycka
Feb 21, 2002·The International Journal of Biochemistry & Cell Biology·Haitham T IdrissSamuel H Wilson
Apr 12, 2002·The Journal of Biological Chemistry·Valérie SchreiberGilbert de Murcia
Jul 5, 2002·The Journal of Biological Chemistry·Tapas K HazraTadahide Izumi
Aug 30, 2002·The Journal of Biological Chemistry·Masashi TakaoAkira Yasui
May 16, 2003·Molecular and Cellular Biology·Satoshi OkanoAkira Yasui
Jun 7, 2003·Nature Cell Biology·Arkady CelesteAndré Nussenzweig
Aug 23, 2003·The Journal of Biological Chemistry·Stéphanie MarsinJ Pablo Radicella
Sep 12, 2003·DNA Repair·Keith W Caldecott
Sep 23, 2003·Nucleic Acids Research·Sherif F El-KhamisyKeith W Caldecott
Nov 26, 2003·Mutation Research·Grigory L DianovSarah L Allinson
Nov 26, 2003·Mutation Research·Geir SlupphaugHans E Krokan
Dec 4, 2003·DNA Repair·Robert S TebbsJames E Cleaver
Dec 31, 2003·DNA Repair·Sarah L AllinsonGrigory L Dianov
Apr 27, 2004·Nucleic Acids Research·Jinshui FanDavid M Wilson

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Citations

Aug 18, 2010·Cellular and Molecular Life Sciences : CMLS·Muralidhar L HegdeSankar Mitra
Sep 17, 2010·Cellular and Molecular Life Sciences : CMLS·Samuel H WilsonMichelle L Heacock
Sep 17, 2011·Cellular and Molecular Life Sciences : CMLS·Celeste BolinJanet Hall
Jun 22, 2010·Analytical and Bioanalytical Chemistry·Elijah J Petersen, Bryant C Nelson
Oct 23, 2010·Cell Death and Differentiation·D De ZioF Cecconi
Feb 5, 2011·Nature Protocols·Keiji SuzukiShunichi Yamashita
Jul 16, 2008·Nature Reviews. Genetics·Keith W Caldecott
Sep 21, 2012·Nature Reviews. Molecular Cell Biology·Lawrence KazakIan J Holt
May 5, 2005·Nature Structural & Molecular Biology·Keith W Caldecott, Anthony Chalmers
Aug 18, 2009·The Journal of Biological Chemistry·Parameswary A MuniandyMichael M Seidman
Oct 19, 2010·The Journal of Biological Chemistry·Rajendra PrasadSamuel H Wilson
Jul 24, 2010·Antioxidants & Redox Signaling·Flurina C ClementHanspeter Naegeli
May 4, 2005·Nucleic Acids Research·Reto Brem, Janet Hall
Jul 21, 2006·Nucleic Acids Research·Oliver MortusewiczHeinrich Leonhardt
Apr 19, 2007·Nucleic Acids Research·Kazunari HashiguchiAkira Yasui
Apr 14, 2010·Nucleic Acids Research·Keiji SuzukiShunichi Yamashita
Mar 15, 2011·Nucleic Acids Research·Oliver MortusewiczValérie Schreiber
Jul 20, 2012·Nucleic Acids Research·Liv KleppaArne Klungland
Feb 15, 2013·Nucleic Acids Research·Grigory L Dianov, Ulrich Hübscher
May 13, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mei-Kuang ChenYin-Chang Liu
Mar 11, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ahmad BesaratiniaGerd P Pfeifer
Oct 29, 2010·Cold Spring Harbor Perspectives in Biology·Giuseppina Giglia-MariWim Vermeulen
Jan 24, 2009·Photochemistry and Photobiology·Roger H BisbyAnthony W Parker
Jul 12, 2012·Photochemistry and Photobiology·Jean CadetThierry Douki
Oct 22, 2008·Molecular and Cellular Biology·Leizhen WeiNatsuko Chiba
Mar 14, 2007·Molecular and Cellular Biology·Natasha IlesKeith W Caldecott

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