In-situ assembly of Ca-alginate gels with controlled pore loading/release capability

Langmuir : the ACS Journal of Surfaces and Colloids
Alena S SergeevaDmitry Volodkin

Abstract

Development of tailor-made porous polymer scaffolds acting as a temporary tissue-construct for cellular organization is of primary importance for tissue engineering applications. Control over the gel porosity is a critical issue due to the need for cells to proliferate and migrate and to ensure the transport of nutrition and metabolites. Gel loading with bioactive molecules is desired for target release of soluble signals to guide cell function. Calcium-alginate hydrogels are one of the most popular gels successfully utilized as polymer scaffolds. Here we propose a benchtop approach to design porous alginate gels by dispersion of CaCO3 vaterite crystals in sodium alginate followed by the crystal elimination. CaCO3 crystals play a triple role being (i) cross-linkers (a source of calcium ions to cross-link gel network), (ii) pore-makers (leaching of crystals retains the empty pores), and (iii) reservoirs with (bio)molecules (by molecule preloading into the crystals). Pore dimensions, interconnectivity, and density can be adjusted by choosing the size, concentration, and packing of the sacrificial CaCO3 crystals. An opportunity to load the pores with biomolecules was demonstrated using FITC-labeled dextrans of different molecular ...Continue Reading

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Citations

Apr 8, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Natalia FeoktistovaDmitry Volodkin
Dec 21, 2016·ACS Applied Materials & Interfaces·Deepak JainPhilippe Barthélémy
Feb 16, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Diana Gabriela SoaresCarlos Alberto de Souza Costa
Mar 16, 2018·Physical Chemistry Chemical Physics : PCCP·A S VikulinaD Volodkin
May 31, 2019·Micromachines·Alena SergeevaDmitry Volodkin
Aug 6, 2016·Macromolecular Bioscience·Philipp T KühnPatrick van Rijn
Nov 15, 2018·Micromachines·Nadezhda G BalabushevichDmitry Volodkin
Sep 9, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ana M FerreiraDmitry Volodkin

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