PMID: 15381723Sep 24, 2004Paper

In situ biofilm formation by multi-species oral bacteria under flowing and anaerobic conditions

Journal of Dental Research
S K FilocheC D Wu

Abstract

An understanding of biofilm behavior of periodontopathic bacteria is key to the development of effective oral therapies. We hypothesized that interspecies bacterial aggregates play an important role in anaerobic biofilm establishment and proliferation, and contribute to the survivability of the biofilm against therapeutic agents. The system developed in this study assessed a multi-species (Streptococcus gordonii, Actinobacillus actinomycetemcomitans, and Fusobacterium nucleatum) biofilm formation under anaerobic and flowing conditions with the use of an in situ image analysis system. The biofilm was comprised of a base film of non-aggregated cells and complex interspecies aggregates that formed in the planktonic phase which rapidly colonized the surface, reaching 58 +/- 9% and 65 +/- 11.8% coverage by 5 and 24 hrs, respectively. Upon SDS (0.1%) treatment of a 24-hour biofilm, substantial detachment (55 +/- 14%, P < 0.05) of the aggregates was observed, while the base film bacteria remained attached but non-viable. Rapid re-establishment of the biofilm occurred via attachment of viable planktonic aggregates.

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Citations

Oct 4, 2011·Lasers in Medical Science·Martin SchneiderAndreas Braun
Sep 26, 2006·Odontology·Jacob M ten Cate
Jul 16, 2008·Journal of Materials Science. Materials in Medicine·A CoughlanM R Towler
Nov 14, 2013·Journal of Environmental Sciences (China)·Zhi-Wu WangJennifer L Morrell-Falvey
Aug 4, 2006·Applied and Environmental Microbiology·Dhana RaoStaffan Kjelleberg
Apr 6, 2007·Journal of Microbiological Methods·Sara K FilocheChris H Sissons
Aug 26, 2009·Oral Microbiology and Immunology·S Y ArslanC D Wu
Oct 29, 2008·Oral Microbiology and Immunology·I SliepenW Teughels
Jan 11, 2012·FEMS Microbiology Reviews·Sivan Elias, Ehud Banin
Nov 18, 2009·Journal of Dental Research·S FilocheC H Sissons
Mar 2, 2016·International Journal of Molecular Sciences·Lorenzo DragoElena De Vecchi
Aug 26, 2004·Oral Microbiology and Immunology·S K FilocheC H Sissons
Aug 26, 2006·Periodontology 2000·Ricardo P TelesSigmund S Socransky
Jul 29, 2005·European Journal of Oral Sciences·Prashant K SharmaHenk J Busscher
Sep 29, 2019·Biofouling·G S OliveiraM C D Vanetti
Jun 27, 2017·NPJ Biofilms and Microbiomes·Jyoti Chhibber-GoelAmit Sharma
Jun 17, 2021·Scientific Reports·Teruyuki HaraTetsuo Ichikawa

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