In-situ birth of MSCs multicellular spheroids in poly(L-glutamic acid)/chitosan scaffold for hyaline-like cartilage regeneration

Biomaterials
Kunxi ZhangJingbo Yin

Abstract

The success of mesenchymal stem cells (MSCs) based articular cartilage tissue engineering is limited by the presence of fibrous tissue in generated cartilage, which is associated with the current scaffold strategy that promotes cellular adhesion and spreading. Here we design a non-fouling scaffold based on amide bonded poly(l-glutamic acid) (PLGA) and chitosan (CS) to drive adipose stem cells (ASCs) to aggregate to form multicellular spheroids with diameter of 80-110 μm in-situ. To illustrate the advantage of the present scaffolds, a cellular adhesive scaffold based on the same amide bonded PLGA and CS was created through a combination of air-drying and freeze-drying to limit the hydration effect while also achieving porous structure. Compared to ASCs spreading along the surface of pores within scaffold, the dense mass of aggregated ASCs in PLGA/CS scaffold exhibited enhanced chondrogenic differentiation capacity, as determined by up-regulated GAGs and COL II expression, and greatly decreased COL I deposition during in vitro chondrogenesis. Furthermore, after 12 weeks of implantation, neo-cartilages generated by ASCs adhered on scaffold significantly presented fibrous matrix which was characterized by high levels of COL I depos...Continue Reading

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Citations

Mar 5, 2016·ACS Applied Materials & Interfaces·Lili WangTong Zhang
Oct 19, 2016·Trends in Biotechnology·Matthias W Laschke, Michael D Menger
Apr 27, 2017·International Journal of Molecular Sciences·Sajjad AshrafSoo-Hong Lee
Feb 21, 2018·Biomedical Materials·Marissa A Gionet-Gonzales, J Kent Leach
Sep 30, 2017·Biomaterials Science·Jie WuJingbo Yin
Feb 25, 2020·Journal of Biomedical Materials Research. Part a·Hongyi LiuYilin Zhao
Apr 28, 2017·Stem Cell Research & Therapy·Yuriy PetrenkoŠárka Kubinová
Feb 17, 2017·Journal of the Royal Society, Interface·X CuiH Zhang
Jun 2, 2017·TheScientificWorldJournal·José Carlos Viana RibeiroVilma Lima
Sep 15, 2020·Clinical Hemorheology and Microcirculation·Yan NieAndreas Lendlein
May 3, 2016·Materials·Lauren EdgarGiuseppe Orlando
Jun 28, 2019·International Journal of Molecular Sciences·Marta Torres-TorrillasJoaquin J Sopena
Nov 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Weijun ZhangJingbo Yin
Mar 19, 2020·World Journal of Stem Cells·Gabriela S KronembergerLeandra Santos Baptista
Aug 6, 2020·Frontiers in Bioengineering and Biotechnology·Dinesh DhamechaJyothi U Menon
Jul 4, 2018·International Journal of Molecular Sciences·Elena DamiaJose Maria Carrillo
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Dimitrios Kouroupis, Diego Correa
Aug 18, 2020·ACS Applied Materials & Interfaces·Zijie XiahouJingbo Yin
Sep 21, 2017·ACS Applied Materials & Interfaces·Pengfei XiaJingbo Yin
Oct 8, 2018·ACS Biomaterials Science & Engineering·Xiangyu LiangJiandong Ding

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