In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts

Arteriosclerosis, Thrombosis, and Vascular Biology
M ShafiqSoo Hyun Kim

Abstract

The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood v...Continue Reading

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Citations

Oct 14, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Muhammad ShafiqSoo Hyun Kim
Oct 28, 2019·Journal of Biomedical Materials Research. Part a·Haoyong YuanZhongshi Wu
Dec 24, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Hong S LuAlan Daugherty
Apr 3, 2020·Nature Communications·Randall J SmithStelios T Andreadis
May 12, 2020·Frontiers in Immunology·Dong-Jin KimSang-Ho Lee
Jan 3, 2019·Vascular Biology·Jarkko P HytönenSeppo Ylä-Herttuala
Nov 1, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anabela MoreiraGareth R Williams
Nov 23, 2021·Frontiers in Bioengineering and Biotechnology·David Durán-ReyFrancisco M Sánchez-Margallo

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Methods Mentioned

BETA
coronary artery bypass
scanning electron microscopy

Software Mentioned

GraphPad Prism
Nikon NIS Elements
origin pro
Image
Pro Plus
image J

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