In situ classification and image cytometry of pelagic bacteria from a high mountain lake (gossenkollesee, austria).

Applied and Environmental Microbiology
J PernthalerR Psenner

Abstract

We describe a procedure to measure the cell sizes of pelagic bacteria after determinative hybridization with rRNA-targeted fluorescently labeled oligonucleotide probes. Our approach is based on established image analysis techniques modified for objects simultaneously stained with two fluorescent dyes. It allows the estimation of biomass and cell size distribution and the morphological characterization of different bacterial taxa in plankton samples. The protocol was tested in a study of the bacterioplankton community of a high mountain lake during and after the ice break period. Cells that hybridized with a probe for the domain Bacteria accounted for 70% of the bacterial abundance (range, 49 to 83%) as determined by 4(prm1),6(prm1)-diamidino-2-phenylindole staining (K. G. Porter and Y. S. Feig, Limnol. Oceanogr. 25:943-948, 1980), but for >85% of the total biomass (range, 78 to 99%). The size distribution for members of the beta subclass of the Proteobacteria shifted toward larger cells and clearly distinguished this group from the total bacterial assemblage. In the surface water layer beneath the winter cover, bacteria belonging to the beta 1 subgroup constituted about one-half of the beta subclass abundance. The mean cell vol...Continue Reading

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Feb 1, 1997·Applied and Environmental Microbiology·J PernthalerR Psenner

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Citations

Jul 2, 1998·Applied and Environmental Microbiology·E Llobet-BrossaR Amann
Oct 31, 1998·Applied and Environmental Microbiology·J PernthalerR Amann
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Jakob Pernthaler, Rudolf Amann
Nov 17, 2006·FEMS Microbiology Letters·Yongqin LiuSijun Huang
Sep 19, 2016·Environmental Monitoring and Assessment·Mustafa NakipogluBulent Icgen
Oct 7, 2004·Applied and Environmental Microbiology·Jakob PernthalerKlaus Jürgens
Sep 9, 2000·FEMS Microbiology Reviews·J Vives-RegoG Nebe-von Caron
May 7, 2003·Applied and Environmental Microbiology·Jakob PernthalerRudolf Amann
Sep 5, 2008·Current Protocols in Cytometry·D MarieD Vaulot
Apr 11, 2001·FEMS Microbiology Ecology·M W. Hahn, M G. Höfle
Mar 3, 2007·Applied Microbiology and Biotechnology·Holger Daims, Michael Wagner
Mar 13, 2016·Bulletin of Environmental Contamination and Toxicology·Bulent Icgen, Fadime Yilmaz
Jan 21, 2006·Environmental Microbiology·Holger DaimsMichael Wagner

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