In Situ Crosslinkable Gelatin Hydrogels for Vasculogenic Induction and Delivery of Mesenchymal Stem Cells

Advanced Functional Materials
Sue Hyun LeeHak-Joon Sung

Abstract

Clinical trials utilizing mesenchymal stem cells (MSCs) for severe vascular diseases have highlighted the need to effectively engraft cells and promote pro-angiogenic activity. A functional material accomplishing these two goals is an ideal solution as spatiotemporal and batch-to-batch variability in classical therapeutic delivery can be minimized, and tissue regeneration would begin rapidly at the implantation site. Gelatin may serve as a promising biomaterial due to its excellent biocompatibility, biodegradability, and non-immuno/antigenicity. However, the dissolution of gelatin at body temperature and quick enzymatic degradation in vivo have limited its use thus far. To overcome these challenges, an injectable, in situ crosslinkable gelatin was developed by conjugating enzymatically-crosslinkable hydroxyphenyl propionic acid (GHPA). When MSCs are cultured in 3D in vitro or injected in vivo in GHPA, spontaneous endothelial differentiation occurs, as evidenced by marked increases in endothlelial cell marker expressions (Flk1, Tie2, ANGPT1, vWF) in addition to forming an extensive perfusable vascular network after 2-week subcutaneous implantation. Additionally, favorable host macrophage response is achieved with GHPA as shown b...Continue Reading

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Citations

Feb 13, 2016·Trends in Biotechnology·Barbara J KlotzFerry P W Melchels
Jun 5, 2018·Stem Cells International·Jeong-Kee YoonHak-Joon Sung
Aug 30, 2020·Bioengineering·Moon Sung KangDong-Wook Han
Feb 1, 2020·Nature Communications·Jung Bok LeeHak-Joon Sung
Jan 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Phuong Le ThiKi Dong Park
Dec 11, 2020·International Journal of Nanomedicine·Que BaiTingli Lu
Jun 19, 2021·Soft Matter·Günnur Onak PulatOzan Karaman
Aug 10, 2021·ACS Biomaterials Science & Engineering·Min Hee KimChien-Chi Lin
May 6, 2017·ACS Applied Materials & Interfaces·Yunki LeeKi Dong Park
Jan 16, 2021·ACS Biomaterials Science & Engineering·Zhe GongChengzhen Liang

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