PMID: 6983909Jan 1, 1982Paper

In situ demonstration of actin in normal and injured ocular tissues using 7-nitrobenz-2-oxa-1,3-diazole phallacidin

Cell Motility
Sheldon R GordonH Rothstein

Abstract

The fluorescent derivative of the actin-binding toxin phallacidin, 7-nitrobenz-2-oxa-1,3 diazole phallacidin, has been used to cytologically demonstrate the presence of actin in lens epithelium, corneal endothelium, and retinal pigment epithelium. In these noninjured tissues, no stress fibers are observed and fluorescence is confined mainly to an area at or near the cell membrane, although some diffuse cytoplasmic staining can also be seen. However, following injury to either the lens epithelium or corneal endothelium of rats and frogs, stress fibers are detected, but only in those cells that migrate into the wound area. Cells on the periphery of each tissue do not partake in would repair and thus maintain their normal appearance. After the tissue has regenerated, stress fibers disappear, and those cells involved in the injury response return to their normal morphology. When rabbit corneal endothelium is placed in tissue culture, stress fibers are observed as the cells migrate away from the initial explant. Upon reaching confluency, these cells spread out and each is surrounded by thick actin-containing bands. Furthermore, they exhibit some stress cables within their cytoplasm. This is in contrast to their appearance in vivo wh...Continue Reading

References

Jun 1, 1977·Die Naturwissenschaften·T Wieland
Jul 1, 1977·The Journal of Cell Biology·S E Hitchcock
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E D Korn
Jun 20, 1978·Annals of the New York Academy of Sciences·M H HeggenessS J Singer
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E WulfT Wieland
Sep 1, 1978·The British Journal of Ophthalmology·A Rahi, N Ashton
Nov 1, 1978·Cell·R O Hynes, A T Destree
Jun 8, 1979·Cell and Tissue Research·B H TohA Fairfax
Apr 1, 1978·Experimental Cell Research·M Kaiho, A Sato
May 1, 1979·Scientific American·E Lazarides, J P Revel
Jul 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R D Goldman
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J W Sanger
Nov 28, 1973·Nature: New Biology·A FagraeusG Biberfeld
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, K Weber
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·T E Schroeder
Sep 1, 1974·Archives of Ophthalmology·M Perlman, J L Baum
Jun 1, 1971·Gut·L J FarrowW D Brighton
Aug 11, 1971·Nature: New Biology·L J FarrowW D Brighton
Jan 1, 1971·International Review of Cytology·C V HardingM Bagchi
Dec 17, 1971·Nature·N Gawadi
Jan 1, 1971·Experimental Cell Research·J F Gierthy, H Rothstein
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M VerderameR Pollack
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L S BarakW W Webb
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L S BarakW W Webb
Jan 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C WillinghamI Pastan
Jul 1, 1981·The Journal of Cell Biology·I M HermanT D Pollard
Aug 1, 1963·The Anatomical Record·N S RAFFERTY
Sep 1, 1964·Experimental Cell Research·H ROTHSTEINR G BLAIKLOCK
Sep 1, 1960·Archives of Ophthalmology·N L MILLS, A DONN

❮ Previous
Next ❯

Citations

Jan 1, 1990·Cell Motility and the Cytoskeleton·S R Gordon, C A Staley
Jul 1, 1994·Journal of Chemical Neuroanatomy·E M Abd-el-Basset, S Fedoroff
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·S R Gordon
Jul 11, 2003·Progress in Retinal and Eye Research·Nancy C Joyce
Jan 1, 1986·The Journal of Cell Biology·G LangangerJ De Mey
Sep 15, 1992·American Journal of Ophthalmology·E K KimH F Edelhauser
Jun 1, 1984·Experimental Eye Research·A R RothD L Scholz
Oct 1, 1983·Experimental Eye Research·M IrelandH Maisel
Jan 1, 1985·Cell Motility·P A DetmersJ Condeelis
Jan 1, 1990·Eye·S J Tuft, D J Coster
Jul 11, 2018·Biomechanics and Modeling in Mechanobiology·S S ShishvanV S Deshpande
Mar 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A I GotliebC Lacey
Jan 1, 1988·Cell Motility and the Cytoskeleton·W Liou, N S Rafferty
Sep 5, 2015·Biomechanics and Modeling in Mechanobiology·A VigliottiV S Deshpande
Jul 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Opas
Oct 1, 1988·Journal of Muscle Research and Cell Motility·H FaulstichJ V Small

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.