In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae

Journal of Biotechnology
Farid Talebnia, Mohammad J Taherzadeh

Abstract

Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5h(-1). The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1h(-1), where the cells lost 75% of their viability measured by colony forming unit (CFU). However, encapsulation increased their capacity for in situ detoxification of the hydrolyzate and protected the cells against the inhibitors present in the hydrolyzate. While the cells were encapsulated, they could successfully ferment the hydrolyzate at tested dilution rates 0.1-0.5h(-1), and keep more than 75% cell viability in the worst conditions. They produced ethanol with yield 0.44+/-0.01 g/g and specific productivity 0.14-0.17 g/(gh) at all dilution rates. Glycerol was the main by-product of the cultivations, which yielded 0.039-0.052 g/g. HMF present in the hydrolyzate was converted 48-71% by the encapsulated yeast, while furfural was totally converted at dilution rates 0.1 and 0.2h(-1) and partly at the higher rates. Continuous cultivation of encapsulated yeast was also investigated on glucose in synthetic medium up to dilution rate 1.0 h(-1). At this highest r...Continue Reading

References

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Citations

Oct 17, 2008·Applied Microbiology and Biotechnology·Jing-Sheng ChengYing-Jin Yuan
Oct 30, 2012·Applied Microbiology and Biotechnology·Johan O WestmanMohammad J Taherzadeh
Sep 3, 2013·Applied Microbiology and Biotechnology·Sofia LalouVictor Nedovic
Jan 12, 2008·Pharmaceutical Research·Sandrine GraffChristine Charrueau
Dec 20, 2013·Environmental Technology·Päivi YlitervoMohammad J Taherzadeha
Oct 31, 2012·International Journal of Molecular Sciences·Johan O WestmanMohammad J Taherzadeh
Jul 17, 2014·Membranes·Päivi YlitervoMohammad J Taherzadeh
Feb 11, 2014·Critical Reviews in Biotechnology·Antonio D MorenoMercedes Ballesteros
Jul 16, 2010·Biotechnology Advances·Solange I MussattoJosé A Teixeira
Jul 28, 2015·Biotechnology Journal·Johan O Westman, Carl Johan Franzén
May 20, 2014·Bioresource Technology·Päivi YlitervoMohammad J Taherzadeh
Aug 3, 2011·Journal of Biotechnology·Päivi YlitervoMohammad J Taherzadeh
Feb 2, 2008·Biological & Pharmaceutical Bulletin·Sandrine GraffChristine Charrueau

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