In Situ Evaluation of Estrogen Receptor Dimers in Breast Carcinoma Cells: Visualization of Protein-Protein Interactions

Acta Histochemica Et Cytochemica
Erina IwabuchiHironobu Sasano

Abstract

The estrogen receptor (ER) functions as a dimer and is involved in several different biological functions. However ER dimeric proteins have not been identified by in situ methodologies. Structured illumination microscopy (SIM) has been recently developed, which enabled the localization of protein and protein interaction. Therefore, in this study, we firstly demonstrated that ERs formed both homodimers and heterodimers in breast carcinoma cell lines using Nikon's SIM (N-SIM). ERα/α homodimers were detected in the nuclei of both ERα-positive MCF-7 and T-47D cells; 23.0% and 13.4% of ERα proteins formed ERα/α homodimers, respectively. ERα/β heterodimers were also detected in MCF-7 and T-47D. Approximately 6.6% of both ERα and ERβ1 proteins formed ERα/β1 heterodimers in MCF-7. In addition, 18.1% and 22.4% of ERα and ERβ proteins formed ERα/β2 heterodimers and ERα/β5 heterodimers in MCF-7, respectively. In addition, by using proximity ligation assay (PLA) in MCF-7, estradiol-induced ERα/α homodimers and ERα/β1 heterodimers were both detected after 15 to 45 min of treatment and at 15 min, respectively. The percentage of total ER proteins could also be determined using N-SIM. By using both methods, it has become possible to evaluate p...Continue Reading

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Citations

Oct 4, 2018·Acta Histochemica Et Cytochemica·Ken Ichi MatsudaMitsuhiro Kawata
Jan 17, 2019·Acta Histochemica Et Cytochemica·Yuki IzawaRei Takahashi
Oct 18, 2018·International Journal of Molecular Sciences·Yasuhiro MikiKiyoshi Ito
Apr 4, 2021·International Journal of Molecular Sciences·Erina IwabuchiHironobu Sasano

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Methods Mentioned

BETA
bioluminescence
fluorescence microscopy
Protein Assay
proximity ligation assay
super-resolution microscopy

Software Mentioned

PLA
SIM

Related Concepts