In situ expression of B7 and CD28 receptor families in skin lesions of patients with lupus erythematosus

Arthritis and Rheumatism
R W DenfeldJ C Simon


To examine the expression of costimulatory molecules of the B7 and CD28 receptor families in active skin lesions of patients with systemic lupus erythematosus (SLE), subacute cutaneous lupus erythematosus (SCLE), and chronic discoid lupus erythematosus (CDLE). The in situ expression of B7-1, B7-2, BB-1, and CD28 was studied by immunohistochemistry, and B7-1 and B7-2 RNA expression was examined by reverse transcription-polymerase chain reaction. Only in lesional skin from SLE, SCLE, and CDLE patients did dermal and epidermal antigen-presenting cells (APC) express B7-1 and B7-2, particularly when in apposition to CD28+ T cells. These B7-1+ and B7-2+ APC bound CTLA-4 fusion protein. In lesional (but not in nonlesional) skin, keratinocytes expressed BB-1. The majority of infiltrating T cells were CD28+. B7-1 and B7-2 RNA were expressed in lesional skin from SLE, SCLE, and CDLE patients; when dermis was separated from epidermis, only faint B7-1 and B7-2 RNA signals were detectable in the epidermis, indicating that dermal but not epidermal cells were the major source of B7-1 and B7-2 RNA. During treatment, both B7-1 and B7-2 protein and RNA expression were reduced. These in situ findings suggest that costimulation via the B7-CD28 pat...Continue Reading


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Related Concepts

CD86 protein, human
Lupus Erythematosus, Subacute Cutaneous
Lupus Erythematosus, Discoid
Lupus Erythematosus, Systemic
Membrane Glycoproteins
Poly(A) Tail
Leukocyte Differentiation Antigens, Human
CD28 Antigens

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