In situ functionalization of wet-spun fibre meshes for bone tissue engineering

Journal of Tissue Engineering and Regenerative Medicine
Isabel B LeonorRui L Reis

Abstract

Bone tissue engineering success strongly depends on our ability to develop new materials combining osteoconductive, osteoinductive and osteogenic properties. Recent studies suggest that biomaterials incorporating silanol (Si-OH) groups promote and maintain osteogenesis. The purpose of the present research work was to provide evidence that using wet-spinning technologies and a calcium silicate solution as a coagulation bath, it was possible to develop an in situ functionalization methodology to obtain 3D wet-spun fibre meshes with Si-OH groups, through a simple, economic and reliable process. SPCL (blend of starch with polycaprolactone) fibre meshes were produced by wet-spinning, using a calcium silicate solution as a non-solvent and functionalized in situ with Si-OH groups. In vitro tests, using goat bone marrow stromal cells (GBMSCs), showed that SPCL-Si scaffolds sustained cell viability and proliferation. Furthermore, high ALP activity and matrix production indicated that Si-OH groups improve cellular functionality towards the osteoblastic phenotype. Using this methodology, and assembling several wet-spun fibre meshes, 3D meshes can be developed, aiming at designing osteoconductive/osteoinductive 3D structures capable of sti...Continue Reading

References

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Citations

Jul 7, 2012·Biomedical Microdevices·Dario PuppiFederica Chiellini
Dec 1, 2012·Biotechnology Advances·Ali TamayolDavid Juncker
Nov 12, 2013·Tissue Engineering. Part a·Filippo Rossi, Martijn van Griensven
Sep 3, 2013·Journal of Tissue Engineering and Regenerative Medicine·João F RequichaManuela E Gomes
Mar 8, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Joao F RequichaCarlos A Viegas
Mar 29, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Dario PuppiEmo Chiellini
Sep 7, 2016·Biomacromolecules·Farrokh SharifiNastaran Hashemi
Apr 27, 2016·Journal of Biomedical Materials Research. Part a·Ana I RodriguesRui L Reis
Nov 18, 2017·Macromolecular Bioscience·Marilyn C McNamaraNicole N Hashemi
Mar 28, 2012·Journal of Tissue Engineering and Regenerative Medicine·Márcia T RodriguesRui L Reis
Apr 18, 2014·Advanced Materials·Nasim AnnabiAli Khademhosseini
Jul 3, 2019·Npj Regenerative Medicine·Thomas Lee Jenkins, Dianne Little
May 10, 2011·Current Opinion in Biotechnology·Márcia T RodriguesRui L Reis

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