In situ gelatinolytic activity correlates with tumor progression and prognosis in patients with bladder cancer

The Journal of Urology
Koji KawamuraHiroshi Ishikura

Abstract

Degradation of the extracellular matrix by malignant tumor cells has an essential role in the process of tumor invasion and metastasis. The 2 gelatinolytic matrix metalloproteinases (MMPs) MMP-2 and MMP-9 are believed to be key enzymes in this process. We investigated the possible relationship between in situ gelatinolytic activity of MMPs and clinicopathological factors in patients with bladder cancer to clarify whether these proteins would be critical for tumor advancement in this disease. We evaluated the intensity of gelatinolytic activity in 25 bladder cancer tissues by film in situ zymography (FIZ). To clarify the MMP(s) responsible for gelatinolytic activity in bladder cancer tissues we examined MMP-2 and MMP-9 expression in bladder tissues by gelatin zymography. MMP expression was also confirmed by reverse transcriptase-polymerase chain reaction and Western blotting. We then investigated the association between MMP expression detected by gelatin zymography and the intensity of gelatinolytic activity determined by FIZ. FIZ demonstrated that all tumor tissues had in situ gelatinolytic activities. There was a statistically significant correlation between the intensity of gelatinolytic activity, and tumor grade, stage, vess...Continue Reading

References

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Citations

Aug 30, 2007·Cancer Metastasis Reviews·Peter C Black, Colin P N Dinney
Jun 29, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·M W RoomiM Rath
Jan 12, 2010·Journal of Dermatological Science·Takuro Kanekura, Xiang Chen
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Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Yoshiyuki MatsuiAlan I So
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May 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yi-Xin RenJia-Ning Zhang
Oct 21, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Thomas Ludwig

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