In situ hybridization analysis of CRABP II expression in sebaceous follicles from 13-cis retinoic acid-treated acne patients

The British Journal of Dermatology
J SitzmannP K Lemotte

Abstract

The aim of this study was to investigate the effects of 13-cis retinoic acid treatment on cellular retinoic acid binding protein II (CRABP II) mRNA expression in sebaceous follicles from acne patients, using in situ hybridization. Biopsies were taken from uninvolved skin areas in close juxtaposition to inflamed comedos before therapy, and at 2-4 or 14-16 weeks of treatment. Paraffin sections were used for in situ hybridization study with riboprobes transcribed from human CRABP II cDNA. After oral treatment with 13-cis retinoic acid, sebaceous glands were reduced in size and atrophic, and the ratio of sebum-free to fully differentiated (sebum-producing) sebocytes was dramatically increased. The CRABP II expression in the sebaceous gland, and to some extent in infundibular structures, was strongly increased compared with the level of expression in the epidermis. The maximum signal was always found in layers of suprabasal sebocytes lacking lipid droplets, but never in the basal layers. These findings indicate a selective activity of 13-cis retinoic acid on CRABP II mRNA expression in the sebaceous glands of acne patients.

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Citations

Jul 1, 1997·Journal of Clinical Pharmacology·T S HindsE M Knight
Feb 15, 2002·Journal of the European Academy of Dermatology and Venereology : JEADV·C C Zouboulis
Jun 21, 2002·Veterinary Dermatology·Rosa A García-FernándezMaria J García-Iglesias
Sep 20, 2006·The Journal of Investigative Dermatology·Christos C Zouboulis
Nov 7, 2008·The Journal of Investigative Dermatology·Amanda M NelsonDiane M Thiboutot
Sep 12, 2007·Veterinary Dermatology·Rosa A García-FernándezMaria J García-Iglesias
Apr 23, 2009·The Journal of Dermatology·Bong Jin HahmDae Hun Suh
Jan 18, 2006·Veterinary Dermatology·Rosa A García-FernándezMaria J García-Iglesias
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Oct 9, 2013·Dermatologic Therapy·Shelbi C Jim On, Joshua Zeichner

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