In situ hybridization analysis of substance P receptor in the rat retina

Experimental Brain Research
A KondohT Sugimoto

Abstract

Substance P receptor is known to provide a principal interface between tachykinin peptides and tachykinin-sensitive cells in retinal circuitry and to produce several physiological functions such as excitation of ganglion cells. We reported results of in situ hybridization analysis of substance P receptor in rat retina using digoxigenin-labeled RNA probes to yield discrete cell labeling. Distinct hybridization signal was present in a great majority of ganglion cells that provide retinal fibers to a central target. It was also present in a subpopulation of amacrine cells. Following optic nerve crush, ganglion cells lost their hybridization signal in a time-dependent manner, while hybridization-positive amacrine cells were persistently seen. From the results, we identified the hybridization message as distinctly localized to two systems, output cells and intrinsic cells in retinal circuitry.

Citations

May 3, 2005·Journal of Pharmacological and Toxicological Methods·Sarah Lilley, Jon Robbins
Nov 28, 2002·Neuroscience·G CasiniN C Brecha
Sep 24, 2004·Neuroscience·E CatalaniG Casini
Sep 8, 2006·Peptides·Eduard SchmidJosef Troger
Apr 15, 2006·The Journal of Comparative Neurology·Theresa Puthussery, Erica L Fletcher
Apr 29, 1998·The Journal of Comparative Neurology·N Cuenca, H Kolb

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