PMID: 8601338Mar 1, 1996Paper

In situ hybridization and immuno-electron microscope analyses of the Us11 gene of herpes simplex virus type 1 during transient expression

Chromosoma
S BesseF Puvion-Dutilleul

Abstract

The distribution of Us11 RNA and of its encoded protein have been investigated at the ultrastructural level in HeLa cells transiently expressing the Us11 gene of herpes simplex virus type 1. In these transfected cells, Us11 protein accumulates at sites identical to those of lytically infected cells, i.e., in nucleoli and in regions of the cytoplasm that contain ribosomes. Us11 RNA and polyadenylated RNA are scattered over the ribosome-rich areas of the cytoplasm. They also accumulate in the nucleoplasm on clustered ribonucleoprotein (RNP) fibrils but also in clusters of interchromatin granules, some of them contiguous to nucleoli. However they are never found in nucleoli. These data reveal the involvement of interchromatin granules in some steps of Us11 mRNA maturation and/or transport.

References

Nov 1, 1978·Journal of Virology·H S MarsdenN M Wilkie
Dec 1, 1975·Journal of Ultrastructure Research·R E NashW Bernhard
Sep 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F Puvion-DutilleulE H Leduc
May 11, 1988·Nucleic Acids Research·A F CookC L Brakel
Apr 1, 1982·Experimental Cell Research·F Puvion-Dutilleul, A Macieira-Coelho
Jun 1, 1993·Molecular and Cellular Biology·V C Bond, B Wold

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