PMID: 8962715Jan 1, 1996Paper

In situ hybridization evidence for a paracrine/autocrine role for insulin-like growth factor-I in tooth development

Growth Factors
B K JosephW G Young

Abstract

Insulin-like growth factor-I(IGF-I) has both metabolic and growth-promoting activities in many cell and tissue types. Although IGF-I is present in serum, it is also thought to have important autocrine and paracrine functions. Immunohistochemistry for IGF-I and its receptor have shown that IGF-I is synthesised locally by the tooth forming cells which exhibit both the IGF-I and the growth hormone receptors. This concept required to be tested by in situ hybridization. Using a digoxigenin-labelled synthetic oligodeoxyribonucleotide probe for IGF-I, we investigated the distribution of IGF-I mRNA in the continuously erupting rat incisor by in situ hybridization. The distribution and intensity of the hybridization signal varied with the developmental stage of the rat incisor. The cells of the apical loop expressed a positive hybridization signal, but the earliest polarised odontoblasts and pre-ameloblasts did not show any positive signal. The onset of enamel secretion was accompanied by a strong hybridization signal in the secretory ameloblasts as well as the odontoblasts. Maturation ameloblasts also demonstrated IGF-I message in their cytoplasm as well as their nuclei. The cells of the pulp and the dental follicle were consistently n...Continue Reading

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Citations

Jan 21, 2010·Annals of Biomedical Engineering·Akiko TonomuraHideaki Kagami
Oct 1, 2011·In Vitro Cellular & Developmental Biology. Animal·Asako MatsumotoAkiyoshi Taniguchi
Sep 12, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·R BuchailleF Bleicher
Jul 8, 2008·Tissue Engineering. Part B, Reviews·Dimitris Nikolidakis, John A Jansen
May 28, 2013·TheScientificWorldJournal·Ning ZhangHang Li
Nov 16, 2013·Cellular and Molecular Life Sciences : CMLS·H Al-KharobiJ Beattie
Oct 30, 2007·Journal of Endodontics·Javier Caviedes-BucheliHugo Roberto Muñoz
Feb 22, 2005·Archives of Oral Biology·Javier CatónMargarita Zeichner-David
Feb 28, 2008·The Angle Orthodontist·Tomohiro FukunagaTeruko Takano-Yamamoto
Oct 27, 2004·Journal of Dental Research·H Werner, J Katz
Nov 2, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tatsuya YamamotoToshihiko Inage
Aug 5, 2009·The Journal of Bone and Joint Surgery. British Volume·J AlsousouK Willett
Sep 28, 2017·Frontiers in Physiology·Steven J BrookesJennifer Kirkham
May 1, 2018·Frontiers in Bioengineering and Biotechnology·Hanaa Esa AlkharobiReem El-Gendy
Aug 11, 2019·Journal of Oral Biosciences·Chisato AizawaHayato Ohshima
Nov 7, 2002·International Journal of Oral and Maxillofacial Surgery·H Schilephake

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