In situ hybridization histochemistry for the analysis of gene expression in the endocrine and central nervous system tissues: a 3-year experience

Journal of Neuroscience Research
B BlochP Böhlen

Abstract

We report our experience in development of the in situ hybridization (ISH) procedure to detect messenger RNAs (mRNAs) coding for various molecules involved in endocrine glands and central nervous system activity, including mRNAs coding for endorphin precursors [preproenkephalin A (PPA), pro-opiocortin (POMC)], vasopressin, and transferrin. Various conditions of fixation and handling of the tissues were tested to establish optimal parameters for mRNA detection. Double-stranded DNA probes labeled by nick translation, synthetic oligonucleotides labeled at their 5' end, as well as single-stranded RNA probes were used, after incorporation of 32P- or 35S-labeled nucleotides. Specific requirements for efficient and reproducible ISH investigations are discussed. Cells expressing the PPA gene in the adrenal medulla and in the brain were detected by ISH. The results show that ISH is as sensitive as immunohistochemistry in detecting peptide-producing cells in the adrenal and that it allows detection of PPA cell bodies in brain in conditions in which they are inconstantly detected by immunohistochemistry. Unilateral destruction of substantia nigra provokes a dramatic decrease in the number of neurons expressing the PPA gene in the contrala...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Brahic, A T Haase
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G R UhlJ F Habener
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B BlochA Kahn
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·M L Pardue, J G Gall
Aug 9, 1969·Nature·H A JohnK W Jones
May 27, 1983·Science·N Chaudhari, W E Hahn
May 19, 1980·Brain Research·J K WamsleyM J Kuhar
Jan 1, 1983·DNA·J H Eberwine, J L Roberts
May 30, 1983·FEBS Letters·R L BeachB W Festoff
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R H Singer, D C Ward
Jun 1, 1981·The Journal of Comparative Neurology·J C FinleyP Petrusz
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M E HarperG F Saunders

❮ Previous
Next ❯

Citations

Dec 1, 1994·Journal of Neuroscience Research·M PlaterotiG Augusti-Tocco
Jan 1, 1992·Virchows Archiv. A, Pathological Anatomy and Histopathology·J Y LiF Peillon
May 20, 1991·Brain Research. Developmental Brain Research·R Guennoun, B Bloch
Jul 15, 1994·Brain Research. Developmental Brain Research·M C MiquelD Vergé
Jul 1, 1989·Brain Research. Molecular Brain Research·C H KuoN Miki
Jul 1, 1989·Brain Research. Molecular Brain Research·S J AugoodA R Crossman
May 1, 1989·Brain Research. Molecular Brain Research·K NoguchiM Tohyama
May 1, 1990·Brain Research. Molecular Brain Research·T HironakaM Tohyama
Jan 1, 1992·Brain Research. Molecular Brain Research·R Guennoun, B Bloch
Feb 1, 1992·Brain Research. Molecular Brain Research·T Araki, M Tohyama
Sep 1, 1992·Brain Research. Molecular Brain Research·T ArakiM Tohyama
May 1, 1992·Brain Research. Molecular Brain Research·C Llorens-cortesJ Epelbaum
Feb 1, 1994·Brain Research. Molecular Brain Research·E KondoM Tohyama
Sep 18, 1990·Neuroscience Letters·C Le MoineB Bloch
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B Droz, J Kazimierczak
Jan 1, 1991·Neuroscience·R TakemuraN Hirokawa
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M KawataY Sano

❮ Previous
Next ❯

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