In situ hybridization of immunoglobulin light chain mRNA in paraffin sections using biotinylated or hapten-labelled oligonucleotide probes

The Journal of Pathology
J H PringleI Lauder


An in situ hybridization technique has been developed for the detection of immunoglobulin light chain mRNA in routine pathology specimens. The method detects kappa or lambda constant region sequences using a cocktail of synthetic oligonucleotide probes labelled with biotin or fluorescein 5-isothiocyanate (FITC) reporter molecules. The probes were labelled at flanking sites chemically by primary amine directed acylation and by 'homopolymer tailing' with terminal deoxynucleotidyl transferase using non-radioactive nucleotide analogues. The mRNA was unmasked in the formalin-fixed tissue sections by digestion with varying concentrations of proteinase K, and the hybrids were demonstrated using alkaline phosphatase with either a streptavidin/biotin based four-stage system or an anti-FITC antibody based detection system. Alkaline phosphatase was visualized using a Fast Red naphthol-capture method and the sections were counterstained with haematoxylin. The results confirm that the method is specific for kappa or lambda mRNA and show that specific mRNAs can be detected in routine formalin-fixed sections using non-radioactive techniques with retention of good morphology. The method reliably detects light chain mRNA in cells expressing sec...Continue Reading


Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
Aug 15, 1985·Analytical Biochemistry·J P CoghlanG W Tregear
Feb 1, 1989·Clinical Immunology and Immunopathology·S L Brown, S L Morrison
May 11, 1988·Nucleic Acids Research·A F CookC L Brakel
Jan 1, 1987·Methods in Enzymology·L M AngererR C Angerer
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P R LangerD C Ward
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G J FreemanH Cantor
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R P PerryJ F Kearney
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R H Singer, D C Ward
Oct 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G S Wood, R Warnke

❮ Previous
Next ❯


Feb 1, 1996·The Journal of Pathology·C A AngelS S Bleehen
Jul 1, 1997·The Journal of Pathology·A M McNicol, M A Farquharson
Dec 28, 1995·Brain Research. Molecular Brain Research·S G VolsenA Gillespie
Jan 1, 1996·European Journal of Cancer. Part B, Oral Oncology·R C JordanP M Speight
Jun 16, 2000·Molecular and Cellular Endocrinology·A FragaleA D'Agostino
Jul 25, 2006·The American Journal of Surgical Pathology·Ken H YoungDennis D Weisenburger
Mar 17, 2006·Applied Immunohistochemistry & Molecular Morphology : AIMM·Cynthia M MagroNeil Crowson
Dec 1, 1994·Histopathology·C A AngelS S Bleehen
Jul 1, 1994·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A F LauritzenH W Nielsen
Mar 1, 1991·Journal of Clinical Pathology·A Warford, I Lauder
Jan 13, 1995·Cell·P H JonesF M Watt
Sep 15, 1993·Cancer·D A HiltonH Nandha
Sep 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C N PalmerI R Phillips
Oct 1, 1993·The Journal of Pathology·K HellR Fischer
May 1, 1995·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S McQuaidG M Allan
Feb 27, 2003·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Rose C BeckEric D Hsi
Sep 13, 2001·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·C MorrisonG J Nuovo
Jun 26, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D G PoweN S Jones

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