In Situ Hydrogel Conditioning of Tissue Samples To Enhance the Drug's Sensitivity in Ambient Mass Spectrometry Imaging

Analytical Chemistry
Xiaowei SongZeper Abliz

Abstract

Ion suppression from the tissue matrix has a severe effect on the mass spectrometry imaging (MSI) of drugs. This problem hinders further applications of MSI in preclinical drug research and development. In this study, an in situ hydrogel conditioning method was developed to enhance the sensitivity of air-flow-assisted desorption electrospray ionization (AFADESI)-MSI. Instead of the traditional washing or digestion treatment in solvent, this method used a solid phase hydrogel to "wash" tissue sections. It was demonstrated that this in situ hydrogel conditioning method improved the drug signal by as much as 2- to 25-fold in MSI, especially for hydrophobic compounds. Furthermore, the obvious dislocation of analytes was not observed. The evaluation of spatial resolution indicated that the amount of dislocation in tissue sections with the hydrogel process was less than the resolution of AFADESI-MSI. The underlying reasons for the MSI signal enhancement were initially investigated. The decreased signal intensities of choline, betaine, and carnitine and the increased intensities of the [M + H]+/[M + Na]+ and [M + H]+/[M + K]+ ratios for drugs in the mass spectra of pretreated tissues provided evidence that this method can reduce the l...Continue Reading

References

Sep 27, 2007·Chemical Communications : Chem Comm·Anthony B Costa, R Graham Cooks
Nov 6, 2007·Journal of the American Society for Mass Spectrometry·Ayanna U JacksonGary J Van Berkel
Nov 30, 2007·Journal of the American Chemical Society·Leiliang ZhengNicholas Winograd
May 13, 2008·Journal of the American Society for Mass Spectrometry·Erin H SeeleyRichard M Caprioli
Sep 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erin H Seeley, Richard M Caprioli
Dec 17, 2010·Chemical Communications : Chem Comm·Allison L DillR Graham Cooks
Feb 22, 2011·Journal of Agricultural and Food Chemistry·Dejuan HuangHuanwen Chen
Mar 19, 2011·Rapid Communications in Mass Spectrometry : RCM·Jiuming HeZeper Abliz
Apr 26, 2011·Journal of Proteomics·Tyler GreerLingjun Li
Jul 31, 2012·Journal of Proteomics·Brendan Prideaux, Markus Stoeckli
Sep 22, 2012·Mass Spectrometry Reviews·Chunping WuDemian R Ifa
Feb 14, 2013·Analytical Chemistry·Glenn A HarrisRichard M Caprioli
Aug 13, 2013·Journal of the American Chemical Society·Bo YanRichard W Vachet
Nov 28, 2013·Analytical and Bioanalytical Chemistry·Shane R EllisRon M A Heeren
Dec 18, 2013·Rapid Communications in Mass Spectrometry : RCM·Jingzhou LiuZhiyang Zhao
Apr 9, 2014·Journal of the American Chemical Society·Livia S EberlinRichard N Zare
Jun 27, 2014·International Journal of Molecular Sciences·Hiroki KannenKunio Awazu
Dec 20, 2014·Analytical Chemistry·Anna NilssonPer E Andrén
Feb 6, 2015·Nature Nanotechnology·Suming ChenZongxiu Nie
Apr 29, 2015·Analytical Chemistry·Tooru KamataHitoshi Tsuchihashi
Jun 13, 2015·Assay and Drug Development Technologies·Adeola ShoboThavendran Govender
May 24, 2016·Analytical Chemistry·Simeon Vens-CappellKlaus Dreisewerd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.