In-Situ Imaging of Azoreductase Activity in the Acute and Chronic Ulcerative Colitis Mice by a Near-Infrared Fluorescent Probe.

Analytical Chemistry
Yang TianChun-Yan Li

Abstract

Azoreductase (AzoR) is an essential reductive enzyme which is closely associated with the intestinal disease such as ulcerative colitis (UC). To date, only a few fluorescent probes for detecting AzoR activity in bacteria or cells have been constructed successfully. It is still challenging to design fluorescent probes for in situ monitoring AzoR in vivo. In this paper, a near-infrared (NIR) fluorescent probe (Cy-Azo) based on hemicyanine is designed and synthesized. The emission of the probe is located at 735 nm in the NIR region, which is favorable for its application in vivo. In addition, Cy-Azo shows high sensitivity to AzoR activity with 17-fold fluorescence enhancement and is particularly selective to AzoR over other enzymes, ions, and amino acids. Meanwhile, a possible response mechanism (the azo group in Cy-Azo is reduced by AzoR and cleaved resulting in the production of Cy-NH2) was proposed and verified by HPLC, MS, and theory calculation. In addition, based on low cell cytotoxicity, Cy-Azo is successfully applied in visualizing the activity of AzoR in two cell lines (HCT116 and HepG2 cells) and three types of bacteria (E. coli, S. aureus, and P. aeruginosa). In particular, due to its NIR emission, the probe can monitor...Continue Reading

References

Oct 5, 2001·The Journal of Biological Chemistry·M NakanishiY Kitade
Jul 24, 2003·Nature Reviews. Immunology·Gerd Bouma, Warren Strober
May 11, 2006·The Journal of Biological Chemistry·Kosuke ItoMasaru Tanokura
Jun 6, 2009·Carcinogenesis·Annemiek M C P JoosenSheila A Bingham
Oct 17, 2009·Chemical Reviews·Junyan Han, Kevin Burgess
May 27, 2010·Chemical Society Reviews·Xiaoqiang ChenJuyoung Yoon
May 3, 2011·Gastroenterology·Jacques CosnesAntoine Cortot
Jul 29, 2011·Chemical Society Reviews·Noël BoensWim Dehaen
Jul 24, 2012·Journal of the American Chemical Society·Lin YuanSasa Zhu
Aug 21, 2013·Chemical Communications : Chem Comm·Arnaud ChevalierAnthony Romieu
Jan 20, 2016·Chemical Communications : Chem Comm·Jianjian ZhangHaixia Zhang
Aug 4, 2016·British Journal of Pharmacology·Ali Ryan
Aug 25, 2018·Chemical Society Reviews·Hong-Wen LiuWeihong Tan

❮ Previous
Next ❯

Citations

May 21, 2021·Chemical Society Reviews·Yurou HuangJuyoung Yoon
Jun 25, 2021·Biosensors & Bioelectronics·Jagpreet Singh SidhuNarinder Singh
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thitima PewklangAnyanee Kamkaew

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.