In situ infiltration of natural killer-like cells induced by intradermal injection of the nucleic acid fraction from BCG.

Microbiology and Immunology
E KuramotoT Tokunaga

Abstract

Intradermal injection of MY-1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)-positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 microgram of MY-1 and lasted for a week. Double immunofluorescence microscopy revealed that the infiltrating GA1-positive cells were all positive for Ly-5, and partially positive for Thy-1.2, but negative for Mac-1, Ia, mu-chain, Lyt-1, Lyt-2, L3T4, and Fc receptor II. They contained neither peroxidase nor nonspecific esterase. The infiltrating cells thus markedly resembled natural killer (NK) cells in their cytochemical characteristics and surface markers. DNase and RNase destroyed the GA1-positive cell-inducing activity of MY-1. These results indicate that the nucleic acid components of MY-1 are responsible for this effect.

References

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Mar 1, 1980·European Journal of Immunology·M KasaiT Tada
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Citations

Aug 17, 2000·Springer Seminars in Immunopathology·S YamamotoT Tokunaga
May 6, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M J SweetD A Hume
Nov 1, 1992·Japanese Journal of Cancer Research : Gann·E KuramotoT Tokunaga
Nov 1, 1992·Infection and Immunity·K L ElkinsA H Fortier

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