PMID: 9161954May 1, 1997Paper

In situ interleukin 5 gene expression in pediatric Crohn's disease

Journal of Pediatric Gastroenterology and Nutrition
G F HankardM Peuchmaur

Abstract

Eosinophils contribute to the intestinal inflammatory infiltrate in Crohn's disease (CD). Eosinophilic infiltration occurs early in Crohn's recurrences, and a release of eosinophil cationic proteins has been observed in active CD. The proliferation, differentiation, and activation of eosinophils are highly dependent on the cytokine interleukin 5 (IL5). In the present study, we used in situ hybridization (ISH) to investigate the expression of the IL5 gene in intestinal specimens from patients with CD. We studied 14 intestinal samples from eight children who had undergone ileocolectomy for advanced CD. The samples were examined for the intensity of the inflammatory infiltrate. Normal pediatric intestine specimens served as controls. In situ hybridization was performed on frozen tissue using radiolabeled IL5 mRNA probes. Positive signal with the IL5 antisense probe was observed within numerous cells infiltrating the specimens involved with CD. The number of IL5-expressing cells correlated with the histological grade of inflammation. Most of the labeled cells were eosinophils, characterized by their bilobed nuclei. Rare IL5-positive cells were detected in the control tissues. No positive signal was obtained with the IL5 sense probe...Continue Reading

References

Sep 26, 1991·The New England Journal of Medicine·D K Podolsky
Jul 1, 1990·The Journal of Clinical Investigation·D EmilieP Galanaud
Oct 1, 1990·Clinical and Experimental Immunology·Y R MahidaC J Hawkey
Jan 1, 1988·The Journal of Experimental Medicine·A F LopezM A Vadas
Oct 1, 1987·The Journal of Experimental Medicine·H M Wang, K A Smith
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K TakatsuA Tominaga
Jan 1, 1995·Virchows Archiv : an International Journal of Pathology·F AutschbachS C Meuer
Feb 1, 1994·The Journal of Experimental Medicine·S DubucquoiM Capron
Feb 1, 1993·European Journal of Immunology·J N BertoliniE M Benson

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Citations

Jan 25, 2003·Journal of Clinical Gastroenterology·Guang-Yu Yang, A Brian West
Nov 5, 1997·Journal of Pediatric Gastroenterology and Nutrition·D BerrebiM Peuchmaur
Sep 29, 2005·Pediatric Research·Sonia MichailFrank Abernathy
May 26, 2001·The American Journal of Gastroenterology·A M LevyK M Leiferman

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